Predicted to enable tRNA dimethylallyltransferase activity. Predicted to be involved in mitochondrial tRNA modification. Predicted to be active in mitochondrion. Human ortholog(s) of this gene implicated in combined oxidative phosphorylation deficiency 35. Orthologous to human TRIT1 (tRNA isopentenyltransferase 1); INTERACTS WITH 2,3,7,8-tetrachlorodibenzodioxine; 2,4-dinitrotoluene; 2,6-dinitrotoluene.