WormMine: AnatomyTerm WBbt:0004364

• Definition: Phasmid neurons, probably chemosensory
• Name: PHBL
• Primary Identifier: WBbt:0004364
• Synonym: lineage name: ABplapppappp

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABplapppappp	ABplapppappp nucleus		WBbt:0002608

1 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 47_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:47_0

211 Expression Patterns

• Primary Identifier: Expr4796
• Remark: Reporter gene fusion type not specified.
• Pattern: cnx-1 was expressed ubiquitously in every blastomere of the embryos up to the gastrulation stage but expression became gradually restricted to the head and tail regions at the comma stage during embryogenesis. During post-embryonic development, cnx-1 was expressed prominently in the H-shaped excretory cell, in the neurons of head and tail, in the dorsal and ventral nerve cords, and in the spermatheca. cnx-1 expression was also observed in the spicules of the male tail. The two head neurons expressing cnx-1 are ASK and ADL, and two tail neurons are PHA and PHB. Therefore, cnx-1 is expressed in head neurons including ASK and ASI chemosensory neurons and tail neurons including PHA and PHB.

• Primary Identifier: Expr4985
• Remark: Picture: Fig 5.
• Pattern: GFP expression was specifically localized to a few cells at the anterior and posterior regions of the worm. At the anterior region, the GFP expression was identified in two amphid neurons (ASG), the pharyngeal intestinal (PI) valve (six cells), one to two neurons in retrovesicular ganglion (RVG), and two neurons posterior to the PI valve. The GFP expression in the posterior region was localized to the two phasmid neurons (PHA, PHB) and the anal valve (four cells). The results indicated that the promoter of CEFT-1 is specifically activated in chemosensory neurons (amphids and phasmid neurons) and valve regions (PI valve and anal valve). Promoter expression was evident through all developmental stages with a highly restricted GFP expression.

• Primary Identifier: Expr4647
• Pattern: Transgenic lines generated with the partial protein fusion construct (for example, ljEx109) expressed TRPA-1:: GFP in the same cells as ljEx107 (see Expr4646), but with some additional cells, including the majority of amphid sensory neurons (for example, ASH, AWA, AWB, ASI and ASK) and the phasmid neurons PHA and PHB.

• Primary Identifier: Expr4648
• Pattern: Transgenic lines generated with the full-length protein fusion construct (for example, ljEx114) expressed TRPA-1:: GFP in the same cells as ljEx107 (see Expr4646), but with some additional cells, including the majority of amphid sensory neurons (for example, ASH, AWA, AWB, ASI and ASK) and the phasmid neurons PHA and PHB. The full-length TRPA-1:: GFP fusion was also expressed in the PVD and PDE in the postdeirid sensilla and the sensory neurons OLQ and IL1. Other neurons in the head and ventral nerve cord also expressed TRPA-1:: GFP.
• Subcellular Localization: The fusion protein was observed at the cilia of sensory neurons, as well as at the cell body.

• Primary Identifier: Expr4511
• Pattern: RGS-3::GFP expression is seen in seven pairs of head sensory neurons (ASH, ADL, AWB, AWC, ASI, ASJ and ASK) as well as PHA and PHB in the tail.

• Primary Identifier: Expr15649

• Primary Identifier: Expr3175
• Pattern: Expressed in ADF, ADL, AFD, ASE, ASG, ASH, ASI, ASJ, ASK, AWA, AWB, AWC, BAG, PHA, PHB, URX, intestine.

• Primary Identifier: Expr8976
• Remark: Picture: Fig 4E to 4H. npr-5 = Y58G8A.4 in this paper.
• Pattern: The npr-5p::RFP transgene was expressed in a subset of amphid neurons (ADF, ASE, ASG, ASI, ASJ, ASK, AWA, AWB) in the inner labial neuron IL2, in the interneurons AIA and AUA, and in the phasmids (PHA, PHB). Outside the nervous system, npr-5p::RFP was expressed in head, neck, and body muscles throughout larval and adult development.

• Primary Identifier: Expr12765
• Pattern: nekl-1p::gfp is expressed almost exclusively in a subset of ciliated cells, with little or no obvious expression in non-ciliated cells. These cells included all of the ciliated cells in the nematode tail (PHA/B, PQR), and a subset of amphid channel cells in the nematode head.

• Primary Identifier: Expr3037
• Pattern: The 5' untranslated region directed expression to a small subset of sensory cells that are ciliated. GFP signal was observed in the multiple ciliated amphid neurons in the head and both ciliated phasmid neurons (PHA and PHB) in the tail. Expression was also detected in other ciliated sensory neurons, including the inner and outer labial neurons and male tail ray neurons. GFP fluorescence was also detected in the midbody PDE ciliated neuron and PQR ciliated tail neuron.

• Primary Identifier: Expr11753
• Remark: genomic

• Primary Identifier: Expr11755
• Remark: Isoform 1b.1

• Primary Identifier: Expr11756
• Remark: Isoform 1b.2

• Primary Identifier: Expr13039

• Primary Identifier: Expr14174
• Remark: Other Strain: OH14363
• Pattern: 3 head sensory neuron pairs, PHA, PHB, glia

• Primary Identifier: Expr3169
• Pattern: Expressed in ADF, ADL, ASH, ASI, ASJ, ASK, PHA, PHB.

• Primary Identifier: Expr3263
• Pattern: Pnphp-4::GFP is expressed in the amphid neurons in the head and the male-specific CEM neurons. Pnphp-4::GFP is expressed in both pairs of phasmids, the male-specific HOB and RnB neurons.

• Primary Identifier: Expr3185
• Pattern: Expressed in ADF, ADL, ASE, ASG, ASH, ASI, ASJ, ASK, PHA, PHB, URX, labial neurons.

• Primary Identifier: Expr15558

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr12196
• Pattern: To determine where lite-1 is expressed and identify likely sites of lite-1 function, the authors generated and examined worms carrying one of four transgenes derived from the wild-type lite-1 locus: a genomic fragment (lite-1 genomic), a transcriptional fusion (lite-1prom::gfp), a C-terminal translational fusion (lite-1prom::lite-1::gfp), and an N-terminal translational fusion (lite-1prom::gfp::lite-1). GFP expression was observed in a total of 29 cells: pharyngeal neurons M1, M4, M5, and MI; non-pharyngeal neurons ASK, ADL, ASI, ASH, AVG, AVB, RIM, ADF, PHA, PHB, and PVT; and non-neuronal cells Hyp3 (hypoderm), AMso (amphid socket cells), and PHso (phasmid socket cells). AVB was observed only with the C-terminal fusion transgene, and RIM and ADF were observed only with the transcriptional fusion transgene. lite-1 expression in AVG and PVT was previously reported.

• Primary Identifier: Expr15598

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018

4 Parents

Definition	Name	Synonym	Primary Identifier
embryonic cell	ABplapppapp		WBbt:0005970
neuron of lumbar ganglion, left.	lumbar left ganglion neuron		WBbt:0005097
Neuron class of two phasmid neurons, type B.	PHB		WBbt:0007808
the left one in the pair of phasmid sensilla.	phasmid left sensillum		WBbt:0005431