WormMine: ExpressionPattern Expr4653

• Pattern: In L4 germ lines, authors also examined SP56, the earliest known marker of sperm differentiation: SP56 antibodies recognize a minor sperm protein in primary, secondary, and mature spermatocytes. SP56 was first seen approximately 6 hr after the molt from L3 to L4 (3 hr, n = 17; 6 hr, n = 14; 10 hr, n = 19), where staining was limited to the proximal-most germ cells. As animals matured through the L4 stage, the SP56 domain expanded distally to include an increasing number of cells. Importantly, FOG-1 and SP56 were not present in the same cells at the same time. Instead, FOG-1 staining preceded SP56 staining in both time and space. For example, the proximal-most germ cells expressed FOG-1 in late L3 and early L4 ; those same cells expressed SP56 in mid-L4 (6 hr), when the FOG-1 domain had moved distally. Authors conclude that in wild-type larvae, FOG-1 is expressed transiently in germ cells destined for spermatogenesis. In wild-type larvae, FOG-1 became easily detectable in the second half of L2. In early L3 larvae, FOG-1 levels increased in the proximal region of most germ lines, and by late L3, FOG-1 staining was strong in that proximal region of all germ lines. FOG-1 remained abundant for approximately the first half of the L4 stage, although its location moved distally within the germ line.
• Primary Identifier: Expr4653
• Remark: No detailed description expression patterns in other life stages.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001481	fog-1	Y54E10A.4	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038