WormMine: ExpressionPattern Expr15997

• Pattern: We found these animals to show widespread, if not ubiquitous expression of CATP-8, including in the epidermis, muscle, pharynx, intestine, and the major neuronal ganglia. Of note, we detected expression in both neurons that were phenotypically affected in catp-8 mutants as well as neurons that were not visibly affected. For example, clear expression of the endogenous tagRFP::CATP-8 reporter was seen in HSN, PVM, and PVD neurons, but also in ALM neurons and others. We found similar expression patterns in transgenic animals expressing GFP under control of the catp-8 5' region immediately upstream of the ATG start codon, where we also saw widespread expression from embryonic stages onwards, including in muscle, intestine, pharynx and other tissues. Collectively, our studies show that catp-8 is widely, if not ubiquitously, expressed albeit at different levels.
• Primary Identifier: Expr15997
• Reporter Gene: We used CRISPR/ Cas9 engineering to insert a tagRFP fluorescent protein at the N-terminus of CATP-8.

12 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
Neuron class of one neuron, posterior ventral microtuble cell, touch receptor.	PVM	lineage name: QL.paa	WBbt:0004086
Anterior lateral microtubule cell, touch receptor	ALML	lineage name: ABarppaappa	WBbt:0003954
Anterior lateral microtubule cell, touch receptor	ALMR	lineage name: ABarpppappa	WBbt:0003953
Neuron, lateral process adjacent to excretory canal	PVDR	lineage name: V5R.paapa	WBbt:0004088
Neuron, lateral process adjacent to excretory canal	PVDL	lineage name: V5L.paapa	WBbt:0004090

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007514	catp-8	C10C6.6	Caenorhabditis elegans

0 Life Stages