WormMine: ExpressionPattern Expr7884

• Pattern: A strong GFP signal is detectable in these animals in a subset of pharyngeal cells. The tat-3::nlsSV40-gfp and tat-3::nlsSV40-gfp-nlsEGL-13 transgenic nematodes also express the reporters in the hypodermis. In addition to the pharynx and hypodermis, expression of the two reporters is also evident in the seam, anchor, distal tip, and adult vulval cells, and at lower levels, in the progeny of the vulval precursor cells and some unidentified cells in the head and tail regions.
• Primary Identifier: Expr7884
• Remark: Picture: Figure 1. The tat-3::nlsSV40-gfp and tat-3::nlsSV40-gfp-nlsEGL-13 transgenic nematodes exhibit essentially the same tissue-specific pattern of reporter expression. In the tat-3::nlsSV40-gfp nematodes, the NLSSV40-GFP reporter fails to significantly accumulate in the nucleus and, under the UV illumination, the nuclei of the fluorescent cells are indistinguishable from the cytoplasm. As a result, identification of cells expressing the reporter is difficult. In contrast, in the same pharyngeal cells with expression of the NLSSV40-GFP-NLSEGL-13 reporter, the difference in intensity between the cytoplasmic and nuclear fluorescence is dramatic, and the fluorescent nuclei are clearly discernable. The NLSSV40-GFP-NLSEGL-13 reporter seems to be more nuclear and to provide a much better contrast in the fluorescence intensity between the nucleus and the cytoplasm than even 4NLSSV40-GFP. Neither reporter is detectable in the hypodermal cytoplasm. The intensity of nuclear fluorescence in the tat-3::nlsSV40-gfp animals is similarly barely above the threshold of detection. In contrast, NLSSV40-GFP-NLSEGL-13 strongly accumulates in the hypodermal nuclei and unambiguously reveals itself in this tissue.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00012360	tat-3	W09D10.2	Caenorhabditis elegans

0 Life Stages