| Pattern | Adult hermaphrodites and males expressed more GLD-4 than animals containing no germline. By immunocytochemistry, GLD-4 was found to be cytoplasmic in the hermaphrodite germline and primordial germ cells throughout embryogenesis. In the late embryo, GLD-4 expression became limited to the germ cell lineage and its localization correlated perfectly with embryonic P granules. GLD-4 was detected throughout embryonic development in the entire P-cell lineage, which gives rise to the two primordial germ cells Z2 and Z3. | Primary Identifier | Expr8623 |
| Remark | Picture: Fig 2. | Subcellular Localization | Subcellularly, GLD-4 localizes also to cytoplasmic structures that are nuclear envelope-associated and the staining pattern is reminiscent of P granules. Indeed, GLD-4 overlaps in colocalization experiments with PGL-1, a molecular marker for P granules. The staining pattern was observed with both C-terminal anti-GLD-4 antibodies and is reduced to background signals in the gld-4(ef15) allele. The P granule association of GLD-4 was less apparent during pachytene, became obvious again in maturing oocytes, and was most prominently visible in developing embryos. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes. | germ line | germline | WBbt:0005784 |
| Germ line precursor cell | Z2 | lineage name: P4.p | WBbt:0004576 |
| Germ line precursor cell | Z3 | lineage name: P4.a | WBbt:0004575 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00014115 | gld-4 | ZK858.1 | Caenorhabditis elegans |
