| Pattern | Immunostaining using an antibody raised against recombinant UNC108 revealed a broad distribution of UNC108 immunoreactivity in the nervous system. | Primary Identifier | Expr8810 |
| Remark | Picture: Fig. 2. | Subcellular Localization | Imaging of a transgenic strain expressing a GFPUNC108 fusion in nine ventral cord motor neurons revealed a distribution similar to that observed by UNC108 immunostaining. Specifically, most neuronal somas had approximately two areas of concentrated UNC108 along with other nonuniform areas of lessenriched UNC108. UNC108 immunoreactivity also coincided with the dense network of axons in the nerve ring. However, imaging larger synapses at higher resolution using both immunostaining and live animal imaging of GFPUNC108 showed that it is largely excluded from the synaptic areas of axons. Within neuronal cell somas UNC108 appeared highly enriched within or surrounding up to several subcellular structures roughly 250500 nm in diameter. Consistent with previous studies of Rab2, these organelles represent the Golgi apparatus because fluorescently tagged UNC108 colocalizes with Golgi markers, and Rab2 concentrates in and near the Golgi by immunoEM (see Expr8808). However, UNC108 immunoreactivity also appeared nonuniformly throughout the cytoplasm. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities. | nervous system | WBbt:0005735 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006833 | unc-108 | F53F10.4 | Caenorhabditis elegans |
