| Primary Identifier | Expr8389 | Remark | Picture: Figure 1D - 1G. |
| Reporter Gene | To examine subcellular localization of EPS-8 within epidermal cells authors inserted GFP into the N-terminus of EPS-8 and expressed it under the control of the epidermal-specific vab-19 promoter. The eps-8 locus generates two isoforms that differ in their C-termini, as a result of alternative splicing: EPS-8A contains the C-terminal actin binding effector domain, whereas EPS-8B does not. Both EPS-8::GFP fusion proteins displayed indistinguishable patterns in the epidermis, and are referred to generically as EPS-8::GFP. | Subcellular Localization | In post-embryonic stages EPS-8::GFP was localized to circumferential bands in epidermis adjacent to muscles, a pattern corresponding to trans-epidermal attachments. EPS-8::GFP also localized to other sites of trans-epidermal attachments. These results indicate that in epidermal cells EPS-8, like VAB-19, is localized to sites of cell-matrix attachments. In pharyngeal marginal cells, Pvab-19-EPS-8B::GFP was localized to the apical (lumenal) ends of attachment structures and not to the basal surface. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Epidermal layer. | hypodermis | epidermis | WBbt:0005733 |
| membranous cell attaches the uterus to the lateral epidermis (seam) and forms a thin laminar process dorsal to the vulva. formed by fusion of eight pi cell progeny and AC. | uterine seam cell | utse | WBbt:0006789 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001330 | eps-8 | Y57G11C.24 | Caenorhabditis elegans |
