| Pattern | the Pvbh-1::GFP::vbh-1::vbh-1 reporter showed high expression in both soma and germline. GFP::VBH-1 expression from line xmSi06 was observed in the cytoplasm of intestinal cells, with a clear enrichment in the apical pole in control animals. Although xmSi06 animals show somatic vbh-1 expression the authors cannot discard that this is an artifact of this transgene. | Primary Identifier | Expr11851 |
| Subcellular Localization | Extruded gonads and embryos from xmSi05 adults (soma and germline expression) were placed on a slide and observed under an epifluorescence microscope to analyze the location of the transgene. The authors have previously reported that VBH-1 is expressed in cytoplasmic foci, perinuclear germ granules termed P granules in germ cells, and diffusely expressed throughout the cytoplasm of all blastomeres [WBPaper00031012]. The GFP transgene showed the same expression as the endogenous VBH-1 in germline cells and embryos suggesting that the expression of the transgene was similar to endogenous VBH-1. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes. | germ line | germline | WBbt:0005784 |
| Nucleated cell which has a diploid set of chromosomes. | somatic cell | FMA:72300 | WBbt:0008378 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006888 | vbh-1 | Y54E10A.9 | Caenorhabditis elegans |
