WormMine: ExpressionPattern Expr1578

• Pattern: In adult hermaphrodites carrying either lag-2 5'::lacZ or lag-2::lacZ(intra), beta-galactosidase was observed in the DTC. In adult hermaphrodites carrying lag-2 5'::lacZ, expression was observed exclusively in the DTC nuclei. Beta-galactosidase was found in 62% (30/48) of the DTCs examined in lag-2 5'::lacZ animals, but it was not observed in the germ line or in any non-gonadal cells of these hermaphrodites. Similarly, in adult hermaphrodites carrying lag-2::lacZ(intra), beta-galactosidase was observed in 100% (42/42) of the DTCs examined. In addition to the consistent DTC staining of lag-2::lacZ(intra) animals, there are beta-galactosidase observed within the germ line of 36% (15/42) of the gonads. This germline staining appeared to extend some distance from the distal tip cell. This germline staining was unexpected. LAG-2:: beta-gal(intra) is predicted to have beta-galactosidase on the intracellular side of the fusion protein, which should reside within the cytoplasm of the distal tip cell. Some variable staining was also seen outside the gonad of lag-2::lacZ(intra) animals: a single uncharacterized cell stained positively with X-gal within the head of 2/21 animals, a few cells were positive in the pharynx of 1/21 animals, and a few uncharacterized cells were positive in the tail of 1/21 animals.
• Primary Identifier: Expr1578
• Remark: Reporter gene fusion type not specified.
• Subcellular Localization: nuclei

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002246	lag-2	Y73C8B.4	Caenorhabditis elegans

0 Life Stages