WormMine: ExpressionPattern Expr10044

• Pattern: Whole-mount immunostaining of transgenic L4 larvae reveals that HENN-1::GFP is expressed broadly in diverse somatic tissues and germline. In extruded gonads of xkSi1; henn-1 hermaphrodites, HENN-1::GFP is detected throughout the germline. Notably, the proximal oocytes show cytoplasmic and intense nucleoplasmic HENN-1::GFP expression. Although nucleoplasmic enrichment is lost following fertilization, HENN-1::GFP is also abundant in embryo, with ubiquitous expression prior to gastrulation. HENN-1::GFP is also expressed throughout the germline of xkSi1; henn-1 males. During sperm maturation, we detect enrichment of HENN- 1::GFP in residual bodies, but we cannot definitively conclude that it is excluded from sperm. henn-1 mRNA levels are lowest in young larva and increase as the germline proliferates, peaking in gravid adult. Germline-deficient glp-4(bn2) adult hermaphrodites show approximately a 50% reduction in henn-1 mRNA levels relative to wild-type, indicating that henn-1 mRNA is expressed in both germline and soma. Embryonic levels of henn-1 are high but decrease rapidly; this pattern suggests that, unlike in zebrafish, henn-1 mRNA may be maternally deposited into the embryo. HENN-1 protein is detectable throughout development and in both hermaphrodite and male adults.
• Primary Identifier: Expr10044

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
Nucleated cell which has a diploid set of chromosomes.	somatic cell	FMA:72300	WBbt:0008378

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00015349	henn-1	C02F5.6	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003