WormMine: ExpressionPattern Expr1642

• Pattern: CGH-1 remained present in somatic and P granules in the early embryo. Embryonic P granules can be detected by PGL-1 staining, and are initially segregated by four cell divisions to a single germline precursor (P4), which forms the two germline precursors Z2 and Z3 at around the 100 cell stage. In the germline, these PGL-1 particles were associated with co-localized CGH-1 staining through the 100 cell stage. CGH-1 staining then disappeared from Z2 and Z3 by the 200 cell stage. CGH-1 was also readily detectable in somatic granules throughout the embryo through the four-cell stage. The latter CGH-1 staining then began to diminish, and was barely detectable at the 28 cell stage and absent in 50-100 cell embryos. No CGH-1 staining was detected at later stages. In intact hermaphrodite larvae and adults, CGH-1 was detectable specifically in the gonad in germline but not somatic cells. Paralleling its mRNA expression, CGH-1 levels were barely detectable in L1 and L2 animals, higher during the L3 stage, and significantly increased in L4 and adult gonads. CGH-1 levels remained modest in proliferating cells at the distal gonad end, where it co-localized with the P granule components PGL-1 and the GLH helicases. In the transition zone, where cells enter meiosis, CGH-1 appeared in additional cytoplasmic granules within the central gonad core. These were referred as CGH-1 somatic granules because they appeared to be maintained in the cytoplasm throughout oogenesis and into early embryogenesis, during which they were not segregated to the germline. CGH-1 remained at high levels in association with each granule type in later stage meiotic cells and oocytes. In contrast, in the male gonad, CGH-1 was readily detectable only in cells that were entering meiosis, in which it was also co-localized with P granules.
• Primary Identifier: Expr1642
• Subcellular Localization: CGH-1 is co-localized with the P granule.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
organ producing either sperm or ova.	gonad		WBbt:0005175
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000479	cgh-1	C07H6.5	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057