WormMine: ExpressionPattern Expr2247

• Pattern: In general, the expression patterns found for the translational fusion constructs were similar to those reported above for the transcriptional fusions. NHX-8::GFP was expressed as expected in the seam cells but was also expressed in the pharyngeal muscles, the pharyngeal-intestinal and intestinal-rectal valve cells, and in cells of the vulva. Expression in these additional cells may have been a result of promoter or enhancer elements in the first intron of the nhx-8 gene that are not present in the transcriptional fusion construct. Alternatively, the shorter promoter used in the translational reporter construct (1.5 versus 4 kb) may lack sequences that normally repress expression in these cells. See Expr2237 for expression pattern observed with transcriptional fusion construct.
• Primary Identifier: Expr2247
• Subcellular Localization: NHX-8 appeared to be associated with intracellular membranes. The NHX-8::GFP protein was distributed in the seam cells in a vesicular pattern that excluded the nucleus, with some underlying signal contributed by weakly fluorescent hypodermal syncitia.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
A group of six equivalent cells forms a tightly constructed `valve` that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine.	pharyngeal-intestinal valve	cardia	WBbt:0005767
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
Intestino-rectal valve	virL	lineage name: ABprpappppp	WBbt:0004511
Intestino-rectal valve	virR	lineage name: ABprpappppa	WBbt:0004510

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003735	nhx-8	Y18D10A.6	Caenorhabditis elegans

0 Life Stages