WormMine: ExpressionPattern Expr15832

• Pattern: Expression analysis of a FLP-3 translational fusion (pflp-3::flp- 3::mCherry) confirmed previous expression analyses of the neuropeptide within male-specific spicule neurons. Transcriptional reporters have shown robust flp-3 expression in the amphid IL1 neurons, as well as the sensory PQR and the male-specific interneuron, CP9, although our translational fusion exhibited no PQR or CP9 expression. Previous studies employed 1-2kb regions of promoter sequence driving GFP expression,while our construct employs a 4 kb region, thereby incorporating further regulatory elements that may restrict expression patterns. By including the full coding sequence in our translational fusion, we have also incorporated the regulatory elements found within the introns of the flp-3 gene.Interestingly, we observed localization of mCherry within sensory cilia of male dorsal and ventral IL1 neurons, as well as in puncta spanning their dendrites, consistent with peptide packing into dense core vesicles. Previous studies have observed dense core vesicles within, and being released from, dendritic arbors, supporting our findings that FLP-3 is packaged within the dendrites of the IL1 neurons. We found an identical expression pattern within the IL1 cilia and dendritic puncta of hermaphrodites as well. In males, our flp-3 construct also exhibited male-specific tail expression in the SPD spicule neurons.
• Primary Identifier: Expr15832

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Inner labial neuron	IL1VL	lineage name: ABalppapppaa	WBbt:0004549
Inner labial neuron	IL1VR	lineage name: ABarapppppaa	WBbt:0004547
Inner labial neuron	IL1DL	lineage name: ABalapappaaa	WBbt:0004738
Inner labial neuron	IL1DR	lineage name: ABalappppaaa	WBbt:0004737
Inner labial neuron	IL1L	lineage name: ABalapaappaa	WBbt:0004553
Inner labial neuron	IL1R	lineage name: ABalaappppaa	WBbt:0004551
Multi-cellular organism that can produce both male and female gametes.	hermaphrodite	hermaphroditic organism	WBbt:0007849

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001446	flp-3	W07E11.2	Caenorhabditis elegans

0 Life Stages