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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr720
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Prominent staining of the entire nervous system, specially the axonal processes emanating from neuronal cell bodies is observed at all developmental stages. Neuronal processes including axonal and dendrites consistently stains brighter than the cell bodies. Staining is detected in the central neurophil (nerve ring) in the head, the ventral cord consisting of motor neurons along the body length, lateral nerve cords, lumbar commissures and neuronal cell bodies in the tail ganglia. Staining is observed in the six sets of touch receptor neurons ALML, ALMR, PLML, PLMR, AVM and PVM. In the head region, neurons and their axonal and dendritic processes in the anterior ganglia, lateral ganglia, ventral ganglion, retro-vesicular ganglion and the nerve ring consisting of axonal fibres from neurons located in the head and tail ganglia are brightly labeled. Neurons and their axonal processes are stained in the tail, which has a pair of bilaterally symmetric lumbar ganglia, a small dorso-rectal ganglion and the pre-anal ganglion at the posterior end of the ventral cord. Besides the major axonal bundle of the ventral nerve cord, the dorsal nerve cord and a set of lateral cords along the body length are also stained. Muscle cells, intestine and hypodermal cells stain weakly. Staining of the mitotic spindles in C. elegans are clearly visible in embryos and meiotic spindles in the germline cells in the gonad of adult hermaphrodites. Spindles are stained more strongly and non-spindle structures. |
Stained in neuronal processes and cell bodies. Spindles are stained more strongly. |
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Expr1773
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kin-29::GFP expression was primarily neuronal, with additional expression in body wall muscle and hypodermal cells. Expression persisted through all stages of postembryonic development. Among neuronal cells, kin-29 was expressed in multiple sensory neurons and interneurons in the lateral, anterior, and lumbar ganglia, as well as in motor neurons in the ventral motor cord. kin-29::GFP colocalizes with odr-1::RFP expression in both the AWB and AWC olfactory neurons. kin-29 was also expressed in the ASH, AFD, ASJ, AWA, ASK, ASG, and ASI sensory neurons. In addition, kin-29 was expressed in the AIY and AIZ interneurons. Additional kin-29-expressing cells were not identified definitively. |
KIN-29::GFP was localized cytoplasmically, and was excluded from the nuclei of most, if not all, cell types. KIN-29::GFP was localized cytoplasmically at all stages of development examined, including in dauer larvae. However, KIN-29::GFP was rapidly translocated to the nucleus upon heat shock, but not upon starvation for 18 hr, addition of dauer pheromone, or in a tph-1 mutant background, although transient nuclear localization in a subset of cell types cannot be ruled out. Heat shock-induced translocation was observed in most, if not all cells, including neurons, muscles, and hypodermal cells. Translocation was reversible; KIN-29::GFP was relocalized to the cytoplasm within an hour upon temperature downshift. |
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Expr1901
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The distribution of signal in transgenic worms was unique and highly restricted with respect to tissue and/or stage of development but did not correspond to the descendants of a particular branch of the cell lineage. Fluorescence was first detectable at the comma stage in cells that divided and appeared to migrate during the 2-fold and 3-fold stages. Neuronal staining was obvious from L1 onward and by early L4 was seen to occur in both the dorsal and ventral nerve chords. During this stage, a strong signal was noted in the developing vulva (most likely the vulE and/or vulF cells). By late L4 an intense GFP signal in the spermathecal valve as well as other vulval and/or uterine structures was evident. Expression in the uv1 and uv2 cells was suggested by the pattern of fluorescence around the vulva. However, the nuclear-localized reporter construct stained more nuclei than can be accounted for by expression in these cells alone. With this construct, nuclear localized signal was observed in all four nuclei of the syncytial spermathecal valve cell. Although GFP fluorescence was seen to be strongest in the late L4 and early adult for the spermathecal valve and vulval/uterine structures previously noted, it was seen to persist throughout adulthood. The M8 cell of the terminal bulb of the pharynx, all six cells of the pharyngeal-intestinal valve, and neuronal cell bodies within the metacorpus and around the isthmus of the pharynx also expressed gly-2p::GFP. At least 37 neurons with cell bodies lying next to the ventral nerve chord were positive for gly-2-directed reporter expression in the adult hermaphrodite, although with widely varying levels of staining. There was also GFP fluorescence present in other neurons associated with the preanal, dorso-rectal, and/or lumbar ganglia. In adult males, expression was similar in non-sexually dimorphic tissues and was also observed in axons that project into rays 2, 3, 5, 6, and either 8 or 9 of the copulatory bursa. |
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Expr13203
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Three rectal glands; Faint expression in head and tail neurons: at least 6 neurons in the lumbar ganglion, at least 20 neurons in the anterior ganglion, at least 20 neurons in the lateral ganglia, 2 neurons in the dorsorectal ganglion. |
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