| Pattern | SPD-5 was detected at centrosomes throughout embryogenesis and during postembryonic meiotic divisions that produce sperm in male nematodes. Paternal requirement for SPD-5 was not detected after raising homozygous spd-5(or213ts) males at the restrictive temperature throughout larval development. SPD-5 was not detectable in mature spermatids. | Primary Identifier | Expr2284 |
| Remark | Two different antisera that recognize the same SPD-5 peptide, and one that recognizes a different SPD-5 peptide, gave identical staining patterns, and no foci of SPD-5 protein were detected in fixed spd-5(RNAi) or spd-5(or213ts) mutants during the first attempt at mitosis. | Subcellular Localization | SPD-5 was first detected in early postmeiotic one-cell stage embryos, prior to pronuclear migration but after the sperm-donated centriole had duplicated to produce two detectable foci of SPD-5 staining. These two foci greatly increased in size prior to and during mitosis. In metaphase, SPD-5 was evenly distributed throughout centrosomes. In late anaphase or telophase, SPD-5 surrounded a central core devoid of both MTs and SPD-5. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00004955 | spd-5 | F56A3.4 | Caenorhabditis elegans |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The stage that begins when a C.elegans individual is fully-developed and has reached maturity. | adult Ce | WBls:0000041 | ||
| The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. | embryo Ce | WBls:0000003 |
