WormMine: ExpressionPattern Expr4759

• Pattern: To better localize cellular staining in worms, authors permeabilized worms and performed whole-mount fluorescent immunohistochemistry. These experiments indicated that Ce-GnRHR was localized to the nucleus of maturing oocytes and intestinal cells, to sperm, pharyngeal muscles, but not other cells such as hypodermal cells. Similar staining of these structures was evident for both the anti-human-GnRHR1 and anti-Ce-GnRHR antibodies. Ce-GnRHR staining clearly illustrates an increase in the size of the oocyte nuclei as they mature along the gonadal arm. Upon fertilization, Ce-GnRHR staining becomes diffuse throughout the developing egg, although staining appears to increase during egg development. Only uniform background autofluorescence was apparent in worms treated with secondary antibody alone. Ce-GnRHR also was detected along the myofilament lattice of the pharyngeal muscles. staining along the three parallel muscles that comprise the pharyngeal musculature beginning at the tip of the head and extending to the pharyngeal bulb, staining of all 8 pharyngeal muscle (pm1 to 8) domains, but not in the gaps between contractile zones of adjacent pharyngeal muscle domains. staining of myofilaments that run radially towards the lumen and which are most obvious in the bulbs. These results indicate Ce-GnRHR is present on the pharyngeal musculature.
• Primary Identifier: Expr4759
• Remark: The specificity of binding of anti-Ce-GnRHR antibody to GnRHR was demonstrated by the lack of staining throughout the worm (including the germline, pharyngeal muscle and intestinal cells) when the antibody was preincubated with its antigen (the C-terminus amino acids 386 to 401).
• Subcellular Localization: Ce-GnRHR was localized to the nucleus of maturing oocytes and intestinal cells, to sperm, pharyngeal muscle.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
Male haploid germ cell.	sperm	spermatozoa	WBbt:0006798

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00018798	gnrr-1	F54D7.3	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003