WormMine: AnatomyTerm WBbt:0005790

• Definition: the space in the center of pharynx which allows the passage of food.
• Name: pharyngeal lumen
• Primary Identifier: WBbt:0005790

0 Children

0 Expression Clusters

3 Expression Patterns

• Primary Identifier: Expr4720
• Pattern: Intestine, weak pharyngeal lumen, head neurons including one in amphid.

• Primary Identifier: Expr23
• Remark: pre-hatched embryo(author) = fully-elongated embryo(curator).
• Pattern: About 6 nuclei in the major nerve ring are seen from hatching onwards. The number decreases to 4 by the onset of adulthood. Up to 8 neural nuclei of the head ganglia stain from the pre-hatched embryo stage to adulthood, a few of the cells also showing axonal process staining. Pre-hatched worms also harbour staining at the pharyngeal lumen.

• Primary Identifier: Expr2601
• Remark: These patterns are specific because MH5 and polyclonal VAB-10A antibodies failed to detect a signal in vab-10(h1356) and vab-10Adeficient embryos. Likewise, VAB-10B antibodies could only detect a weak signal in the intestine and pharynx of mid-staged vab-10B(mc44) and vab-10(h1356) embryos. Although it cannot be excluded that VAB-10B antisera recognize a cross-reacting protein, VAB-10B is likely to be expressed in the pharynx and the intestine because surviving vab-10B(mc44) larvae had an abnormal intestine.
• Pattern: In embryos, VAB-10A and VAB-10B antibodies first detected a signal at the basal and apical plasma membranes of dorsal and ventral epidermal cells, soon after the onset of differentiation. As these cells became thinner during morphogenesis, staining appeared as four longitudinal rows and progressively evolved into circumferentially oriented bands located above muscle sarcomeres. VAB-10A was also detected basally and apically in the pharynx and along mechanosensory axons. VAB-10B was also present in the pharynx lumen, intestine lumen, nerve ring, and in body wall muscles and somatic gonad of larvae.
• Subcellular Localization: The circumferential bands formed by VAB-10A matched those formed by IFs at all stages. VAB-10A staining strictly colocalized with myotactin in adults, but only partially in young larvae. The parallel bands formed by VAB-10B are interspersed with those formed by VAB-10A and extend beyond the area of muscle epidermis contact. The VAB-10B bands corresponded to the regularly spaced shallow furrows that pattern the cuticle and separate annuli ridges. Immunoelectron microscopy confirmed that VAB-10A (but not VAB-10B) is restricted to FOs. VAB-10A was exclusively detected in the epidermis in areas dense in filaments, a characteristic of FOs. Using the VAB-10B K22 antibodies, gold particles were observed in the epidermis adjacent to body wall muscles and in sarcomeres. Although the number of particles is low, quantitative analysis indicates that staining is specific. Thus, VAB-10B appears to have a disperse distribution in the epidermis and muscles.

0 Life Stages

2 Parents

Definition	Name	Synonym	Primary Identifier
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Anatomical structures that exist outside of cells; non-cellular.	extracellular component		WBbt:0005732