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WS294

Intermine data mining platform for C. elegans and related nematodes

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Anatomy Term :

Definition  Preanal hypodermis Name  hyp12
Primary Identifier  WBbt:0004376 Synonym  lineage name: P12.pa

1 Children

Definition Name Synonym Primary Identifier
nucleus of pedigree P12.pa P12.pa nucleus   WBbt:0002096

4 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Single-cell RNA-Seq cell group 30_1 expressed in hypodermis. scVI 0.6.0 WBPaper00065841:30_1
  Single-cell RNA-Seq cell group 2_0 expressed in hypodermis. scVI 0.6.0 WBPaper00065841:2_0
  Single-cell RNA-Seq cell group 82_1 with unidentified tissue expression pattern. scVI 0.6.0 WBPaper00065841:82_1
  Single-cell RNA-Seq cell group 82_0 with unidentified tissue expression pattern. scVI 0.6.0 WBPaper00065841:82_0

35 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
Picture: Figure 1.   Expr8361 GFP expression initiated in the early gastrula. Robust expression of Prncs-1::GFP was observed in the midgut (E cell lineage) starting at the 28-cell stage and continuing into adulthood. By the comma stage, fluorescence was also visible in the embryo periphery in cells that give rise to hypodermis. In L1 larva and subsequent stages, strong expression of GFP was seen in hypodermal cells, including Hyp 7 syncytium and head and tail hypodermis. The expression pattern was identical in hermaphrodites and males, but adult hermaphrodites displayed fluorescence in vulval epithelium. Expression was absent in seam cells, nervous system, and pharynx. The Prncs-1::GFP reporter showed increased expression during starvation. Although fluorescence intensity was enhanced under starved conditions, the spatial expression pattern was unchanged. Expression of the Prncs-1::GFP transgene was also enhanced in males. An ~2.5-fold increase in rncs-1 expression in total RNA prepared from wild-type, well fed males, compared with hermaphrodites.  
    Expr4930 strong bwm, vul, anal, rare ant pharynx, bwm precursors, tail hyp, neurons; early embryonic bwm at least by horseshoe stage  
    Expr4344 qua-1pro::GFP was found to be expressed in the hypodermal cells covering the whole body from the tip of the nose to the tip of the tail spike, but not in the lateral hypodermal cells, i.e., the seam cells. Furthermore, expression was seen in the excretory duct and pore cells from threefold stage embryos to adults. However, in adults the GFP intensity appears weaker than in larvae. In L1 larvae, qua-1 is expressed in two, sometimes four, cells of the anterior as well as the posterior of the intestine and a rectal epithelial cell. In addition, transient expression was observed in the P cells in L1 in the ventral side of the animal and in a few sensilla support cells in the head. In adults, qua-1pro::GFP is transiently expressed in a few cells in the head that remain to be identified.  
    Expr4651 GFP driven by the lon-2 promoter (3 kb of its upstream sequence) was expressed strongly in the intestine, most prominently in the most anterior and posterior cells. Expression in these regions was observed throughout development, beginning in the embryo and continuing into adulthood. Very weak fluorescence was also seen in hypodermal cells in the head and tail.  
    Expr3695 Fluorescence was observed in epithelial cells that synthesize cuticle. Expressed in the hypodermis, including the major body syncytium, hyp7, and hypodermal cells in the head and tail. A pulse of fluorescence was observed in the hypodermis prior to molting. Fluorescence from mlt-8p::gfp-pest was first detected approximately 3 h before the L1/L2 molt, or 13 h after hatchlings synchronized by starvation were fed and incubated at 25 centigrades. The intensity of fluorescence increased until lethargus and then decreased rapidly, such that GFP was barely detectable just 2 h after molting. When monitoring individual transgenic larvae over the course of development, fluorescence from mlt-8p::gfp-pest was observed from 65 +/- 2% to 90 +/- 2% of the duration of each larval stage. The mlt-8 reporter was expressed, in larvae, in a single posterior neuron that remains to be identified. Expression of the gfp fusion genes was never detected in the hypodermis of gravid adults that no longer molt.  
Picture: Fig 5G, 5H, 5I.   Expr9039 The expression of nhr-206 GFP reporter transgenes starts during the comma stage of embryogenesis, and is initially seen in four unidentified cells localized in the head region. By the 2-fold stage, embryonic expression is observed in the pharynx with weaker expression in intestine. This expression pattern continues throughout all larval stages and in adults with pronounced anterior pharyngeal expression. The reporter genes were also strongly expressed in rectal gland cells, the anal sphincter, and in epidermal cells in the tail. Weaker expression was also observed in the vulva and spermatheca. In males, expression was visible in male specific neurons of the tail and rays.  
Picture: Fig 5J, 5K, 5L.   Expr9040 The expression of nhr-208 GFP reporters started in embryos at the 1.5-fold stage within the pharynx, intestinal sphincter, and epidermal cells in the tail. By the 3-fold stage of embryogenesis, additional expression was observed in rectal gland and surrounding cells. During all larval stages, strong expression of the transgenes was visible in pharyngeal and unidentified head neurons, the pharyngeal-intestinal valve cell, the posterior part of the intestine, the intestinal sphincter, two rectal gland cells, the intestinal-rectal valve cell, and the epidermal hyp10 cell. In males, the expression was seen in several rays (6-8) and other male specific neurons.  
Picture: N.A.   Expr8910 Expressed in phyaryngeal muscle, marginal cells, all intestinal cells, seam (strong), other hypodermis (weak), arcade cells, spermatheca, vulva and rectal epithelial cells,  
    Expr1396 The earliest detectable expression of UNC-115::GFP was in neurons and epidermis at about 300 min postfertilization, when the embryo begins to elongate, and axons begin to grow. UNC-115::GFP and the shorter fusion GFPS was expressed in most or all neurons throughout development. UNC-115::GFP expression was also observed in non neuronal cells, including the epidermal syncytium hyp7, the head and tail epidermal cells, the excretory canal cell, the pharynx, and the developing vulva, but not in the lateral epidermal seam cells or the ventral epidermal P cells. The earliest detectable expression of UNC-115::GFP was in neurons and epidermis at about 300 min postfertilization, when the embryo begins to elongate, and axons begin to grow. UNC-115::GFP and the shorter fusion GFPS was expressed in most or all neurons throughout development. UNC-115::GFP expression was also observed in nonneuronal cells, including the epidermal syncytium hyp7, the head and tail epidermal cells, the excretory canal cell, the pharynx, and the developing vulva, but not in the lateral epidermal seam cells or the ventral epidermal P cells. UNC-115::GFP protein was present uniformly in neuronal cell bodies and processes and was excluded from nuclei. The protein was also present at high levels in the growth cones of developing axons as they extended to their targets and in cell-cortex-associated plaques along the excretory canals as well as plaques at the junctions of epidermal cells.
Integrated transgenic line not described in the article.   Expr1416 When transgenic animals carrying pTG96 on an extrachromosomal array (kuEx75) were examined, the fusion protein, as judged by fluorescence of GFP, was observed tightly localized to the nuclei of most cells. SUR-5 appeared to be expressed in the VPCs. Cell types that express this fusion protein include neurons, hypodermis, Pn.p cells, body muscles, many cells of the pharynx, and a few cells of the somatic gonads. Cells that do not display the fluorescence include B, F, K , K.a, K.p, hyp3, the germ line, and the excretory duct cells. In nonmosaic animals, the intensity varies among the cells. The intestinal cells and excretory cells are almost always very bright, whereas neurons are almost always fainter. Uterine cells and many of the cells derived from the M cell are very faint and often difficult to see. The SUR-5GFP fusion proteins are expressed in all stages of C. elegans development. The earliest expression is at the 100- to 150-cell embryonic stage, and the fusion proteins are expressed throughout development from that stage on. The same expression pattern is seen when this array is integrated into one of the chromosomes. pTG96_1 is still localized in the nuclei of most cells. The expression pattern is the same as that seen from the array containing pTG96 (with NLS), but the nuclear localization is not as tight, and there appears to be some diffusion of SUR-5GFP proteins from the nucleus to the cytoplasm. Both constructs are expressed in nuclei; and a relatively small amount of SUR-5GFP fusion protein from pTG96_1 is detected in cytoplasm.
    Expr2893 Beta-Galactosidase expression was observed in many cells, including gut, hypodermal, and other cells within the developing embryos. However, because of the intense staining in some parts of the developing embryos, the identification of the many stained cells was not possible. In post-embryonic stages, beta-galactosidase staining was present in the hypodermal cells of all larval stages mature gravid hermaphrodites (710 days after L4 adult hermaphrodite). Beta-Galactosidase staining was also detected in intestinal cells. The cpz-1:lacZ expression in all stages was specifically detected in the large hypodermal syncytium covering most of the worm (hyp7), and in the hypodermal cells of the head (hyp4 and hyp6) and occasionally in hypodermal cells in the tail. Beta-Galactosidase staining was also present in few pharyngeal cells of the adult worms but not in the pharyngeal cells of larval stages. Expression of cpz-1:gfp translational construct was detected in all developmental stages except embryos. The cpz-1:gfp expression was restricted to the hypodermis, with additional expression in the pharynx and the gonad only in the L4 and adult worms stages. The specific expression in the pharynx of adult stages was similar to that observed in cpz-1:lacZ transgenic worms. There was no embryonic expression of the gfp construct.  
Clone: pUL#IAH10A2   Expr7481 Strong consistent expression was seen in all four independent lines examined, but all were very mosaic and three least mosaic lines were retained. Expression was extensive and included nerve cells in the head, tail and ventral nerve cord, strong expression in the excretory cell, occasional weak expression in body wall muscle cells, adult tail hypodermis, the vulva, coelomocytes, pharynx and intestine, particularly anterior and posterior intestine. Expression was particularly strong in the elongating and fully elongated embryo, but expression starts at the comma stage. Expression through larval stages and through to adult is also quite strong.  
Data observed from, atIs13, atEx32 and atEx35.   Expr970 In comma to 1.5-fold stage embryos, the nhr-25::GFP are expressed in the V cells, P cells and hyp7. Expression also observed in the head and tail hypodermal cells of embryos. The earliest expression is at ~250-300 min post fertilization. atEx32 and atEx35 L1 animals exhibited consistent reporter expression in the hyp7 and P cell nuclei. The P cell expression was very strong at hatching. GFP expression in the P cell and hyp7 decreased during mid-L1, but frequently increased late L1. At hatching no expression in observed in the V cells. In mid L1 the V cells divided and the anterior daughters begin to express GFP as they join the syncytium. Strong expression is also seen in the head and tail hypodermal nuclei of L1 larva. Expression in the hypodermal nuclei of older larva stages is similar to that in the L1. GFP expression decreases markedly in adults. GFP is also observed in other ectodermal cells. In L1, expression is observed in the G2(excretory pore) cell and in a cell tentatively identified as the W neuroblast. In older larva, expression continues in G2 but disappears in the W lineage once the cells divides to generate neurons. Beginning in L2, GFP is expressed in the region around the rectum. Expression is also occasionally observed in the anterior pharynx, in the nuclei with positions consistent with those of the pharyngeal epithelial cells. nuclei
Clone: pUL#JRH/AG6   Expr7686 Expression is first seen in comma stage embryos as several cells in the posterior. From late embryo to L3 larva can see expression in the ventral half of the very tip of the tail hypodermis?  
    Expr1164 Transgenic animals that carry this construct show GFP expression in a variety of tissue types. GFP expression is observed in the intestine, and the posterior cells express GFP more intensely than the remaining intestinal cells. Other cells include the rectal epithelial cells, the pharynx, the somatic gonad, and vulval hypodermal cells. In addition, the IP3 receptor is expressed in hypodermal cells of the tail, rectum, and head. Pharyngeal expression is restricted to the muscles of the metacorpus, isthmus, and the anterior portion of the terminal bulb (m4, m5, and m6). This construct was only expressed in neurons LUA and PDA. GFP is expressed in the gonad sheath cells, spermatheca, spermathecal valve, and uterine sheath cells. GFP is expressed in the vulval hypodermal cells.  
Lineage expression: sexmyoblasts and descandents.   Expr1596 LIN-29 was detected in many non-hypodermal cells in the head, tail and vulval region of the developing hermaphrodite. In the head, LIN-29 accumulates in cells of the pharynx and in a subset of neurons. In the tail, LIN-29 accumulates in the rectal cells B, F and U. LIN-29 also accumulates in the sex myoblasts and their progeny, in the distal tip cells, the anchor cell, and in many vulval cells. Although the accumulation of LIN-29 in the hypodermis is restricted to the L4 stage, accumulation in several of these other cell types is not. For example, the accumulation of LIN-29 in the anchor cell and the distal tip cells occurs during the L3 stage. In addition, many, if not all, of the cells that make up the pharynx contain low levels of LIN-29, beginning in the L1 stage and extending to the adult stage. The anti-LIN-29 antisera recognized a nuclear antigen in lateral hypodermal seam cells in wild-type C. elegans. The anti-LIN-29 antibodies revealed a differential pattern of lin-29 protein accumulation during development. LIN-29 was not detected in hermaphrodite hypodermal nuclei prior to the L4 stage. Although it is possible that LIN-29 is distributed diffusely throughout the hypodermal cytoplasm during the L3 and younger stages, there are no difference detected in hypodermal cell staining when these animals were incubated with secondary antibody alone, relative to animals incubated with both primary and secondary antibodies. The earliest LIN-29 accumulation in lateral seam cell nuclei was shortly after their final division, during the L3- to L4-molt. LIN-29 accumulated in these hypodermal nuclei during the L4 stage, and remained detectable in the adult animal. At approximately the same time, LIN-29 was detected in the hypodermal nuclei of the head (hyp1-hyp6), tail (hyp8-hyp12), and the large hypodermal syncytium covering most of the animal (hyp7). The accumulation of LIN-29 in hyp7 was typically observed following accumulation in the seam, and the signal was usually less intense. In summary, LIN-29 accumulates stage-specifically, beginning during the L4 stage and persisting into the adult stage, in all hypodermal cell nuclei of the worm. LIN-29 was also detectable in late stage gravid adults, at a time when lin-29 mRNAs are greatly reduced in abundance. nuclei
Picture: N.A.   Expr8929 Expressed in all intestinal cells and tail hypodermis.  
Picture: Fig 6, Fig 7.   Expr8861 SMF-3::GFP was mostly observed all along the intestine, with a weaker expression in the most proximal and distal regions. A weak epidermal expression in hyp1 to 6, hyp7 and hyp8 to 12, and in head and tail neurons was also seen. The GFP signal was detected from late embryogenesis to adult stage, with generally higher expression levels in young larvae (L1 stage). SMF-1::GFP and SMF-3::GFP were localized at the apical plasma membrane in all epithelia in which they were expressed.
Picture: Figure 4.   Expr8514 Expressed in Head muscle/hypodermis, tail muscle/hypodermis, head, tail and vulva neurons, Nerve cord.  
Picture: Figure 1.   Expr9130 APL-1::GFP fluorescence is detected in the cell bodies and processes of nerve ring interneurons and the ventral cord. apl-1::gfp is also expressed in ocket cells and amphids present in the head. Strong expression is seen in junctional cells such as the pharyngeal intestinal valve, which tethers the pharynx to the intestine, and the uterine seam junction in adults, which provides the structural connection between the epidermis and the uterus. APL-1 can be weakly detected in many epidermal epithelia including hyp7, the hypodermal syncitium surrounding the worm, as well as vulval cells, rectal valve cells, pharyngeal arcade cells, and tail hypodermis. Expression is prominent in the excretory cell, a long H-shaped cell implicated in fluid balance. APL-1 was notably absent from body wall muscle and intestine. APL-1::GFP fluorescence is detected in the cell bodies and processes of nerve ring interneurons and the ventral cord.
    Expr3042 In the nas-37p::gfp-transformed animals, GFP was observed to be expressed in the hypoderma cells except the seam cells and in the rectal gland cells throughout the larval stages. When the -879 to +5 region instead of the -3679 to +5 region of nas-37 was used, a faint fluorescence of GFP was detected in the hypodermal seam cells but not in the other hypodermal cells. Therefore, it is thought that a DNA motif(s) essential for expression is located in the -3679 to +879 region of the nas-37 gene.  
Other strain-- UL765   Expr142 This expression pattern is observed from 3-fold embryo to adult stages and has numerous components. Expression is seen in hypodermal cells of the head and tail regions, and occasionally in posterior intestinal nuclei. Pairs of symmetric neuronal nuclei around the pharyngeal isthmus express, as do cells of the pharyngeo-intestinal valve. Cells of the vulval hypodermis (possibly VulC or VulD) also express. This pattern is highly mosaic and not all components are seen in all worms.  
This fusion gene was able to rescue the Nrf and pale egg phenotypes of nrf-6 mutant animals.   Expr1214 Expressed strong GFP fluorescence in hyp 3 and hyp 5, the most anterior cells in the hypodermis, and in the intestine. This expression began at the L1 stage and continued throughout adulthood. Weaker fluorescence was also observed in other hypodermal cells throughout the animal, particularly in later stages of development.  
Reporter gene fusion type not specified.   Expr1215 Expressed strong GFP fluorescence in hyp 3 and hyp 5, the most anterior cells in the hypodermis, and in the intestine. This expression began at the L1 stage and continued throughout adulthood. Weaker fluorescence was also observed in other hypodermal cells throughout the animal, particularly in later stages of development.  
    Expr3123 Animals transformed with mfb-1p::gfp exhibit GFP fluorescence in the late embryo and through the larval and adult stages, strong expression in the head and tail ganglia, the ventral nerve cord, the tail hypodermal cells and the intestine, weak expression in some lateral neurones, seam cells and body wall muscles in some lines, and no expression in the pharynx and distal tip cells. MFB-1::GFP was prominently detected in some head and tail ganglia, weakly in the ventral nerve cord, but little or not at all in the hypodermis, intestine, seam cells and muscles. In the head and tail ganglia, MFB-1::GFP was preferentially localized to the nuclei.
    Expr3750 Anti-FUS-1 antibody first detects FUS-1 in a punctate pattern within the gut cells of embryos at approximately the comma stage (~6 hr pf); it is undetectable in epidermal cells at this early stage. FUS-1 is also detected in the excretory cell and the apical membrane of gut cells starting later in embryogenesis. In the epidermis, the protein is first conspicuously detected in 2-fold stage embryos shortly after most epidermal cell fusions occur. Initially, FUS-1 expression is seen at approximately equal levels throughout the dorsal, ventral, and anterior epidermal cells; it is specifically excluded from the lateral seam cells. FUS-1 remains virtually undetectable in seam cells, which neither fuse in wild-type embryos and fus-1 mutants nor express EFF-1. As the embryo develops, FUS-1 levels increase in these epidermal cells, where it concentrates almost exclusively on the apical membrane and some colocalizes with apical junctions.
Other strains-- UL870, UL874   Expr426 Diffuse non-nuclear expression is seen in older larvae and adults in three distinct components, i.e. the excretory cell and canals, the posterior hypodermis, and the uterine wall (ut2 toroidal cells). This gene has similarity to amino acid transporters.  
    Expr3966 HPL-1 expression was observed in the nuclei of most, but not all cells of larvae and adults. Expression appeared to be particularly strong in unidentified neuronal and hypodermal nuclei of the head and tail region, consistent with microarray studies showing that the expression profile of HPL-1 correlates well with the expression of neuronal enriched genes. Authors also occasionally observed very transient HPL-1::GFP expression in the germline. nuclei
    Expr3043 In the nas-36p::gfp-transformed animals, GFP was detected in the hypodermal cells at less intensity than in the nas-37p::gfp-transformed animals but was not detected in the rectal gland cells. GFP was also detected in the hypodermal seam cells in L4 larvae and young adults. In old adult hermaphrodites, GFP was expressed in vulva, but was not detected in the hypodermal cells for both the fusion genes. (See Expr3042.  
    Expr3690 Fluorescence was observed in epithelial cells that synthesize cuticle. Expressed in the hypodermis, including the major body syncytium, hyp7, and hypodermal cells in the head and tail. Expression of qua-1p::gfp in the hypodermis intensified prior to molting. Expression of the gfp fusion genes was never detected in the hypodermis of gravid adults that no longer molt.  

0 Life Stages

2 Parents

Definition Name Synonym Primary Identifier
descendent cell of P12, posterior P12.p   WBbt:0006900
primary cell type that forms the hypodermis. hypodermal cell   WBbt:0007846