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Picture: Fig. 7. |
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Expr4376
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ceGAT-1 is expressed in all of the GABA-ergic neurons. These GFP-positive neurons include the VD and DD neurons in the ventral cord, the RMED, RMEV, RMEL, RMER, AVL, and RIS neurons in the head area and the DVB neuron in the tail region. There are two additional GFP-positive neurons in the tail region. These two neurons are PVQR and PVQL. The identity of these GFP-positive neurons were confirmed by epifluorescence microscopy and by the location of the neurons as revealed by a combination of Nomarski-differential interference contrast microscopic observation and 4',6-diamidino-2-phenylindole nuclei-staining method. This expression pattern is evident from the early larva stage through the adult stage. An identical expression pattern was observed with at least 10 transgenic animals. |
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Expr15558
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15586
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Expr15651
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Expr15589
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Expr13158
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Expr15591
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Expr15598
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Picture: Fig 3. |
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Expr8850
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Neuronal Expression: AVA, AVB, AVE, PVC, AIB, AUA, AVG, RIB, RIC, SAA, SIA, SIB, RIF, RIM, RMD, RME, SMD, DA, DB, VA, VB, M5, NSM, MC, I3, MI?. Non-neuronal Expression: rectal epithelium, body wall muscle, spermethecae, vulva muscle. |
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Expr15604
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr15608
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Expr15611
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Expr8203
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Expressed in DA, VB, AS, DB, DD, HSN, VC4 & 5, AIY, head neurons, muscles, intestine. acr-14::GFP is expressed in RME in the head and in ventral nerve cord (VNC) motor neurons. |
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life_stage summary : postembryonic |
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Expr8
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Post-embryonic expression. L1-L4, single ventral cell in middle of the second bulb of the pharynx. L1, single cell in dorsal midline near nerve ring, L2-young adult, 1 dorsal and 1 ventral cell near nerve ring. L2/L3 cell in dorsorectal ganglion. |
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Expr3136
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C33G8.5 showed medium to strong expression in scattered nonchemosensory neurons.5 |
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Expr16053
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A functional transcriptional reporter for CKR-1 revealed strong expression in many neurons and weak expression in the intestine. In the nervous system, ckr-1 expresses in the head and ventral cord neurons. CKR-1-expressing neurons include head motor neurons SMD and RME , interneurons AIB and RIM, peptidergic neurons RIS, and body motor neurons A, B, and D. |
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Expr10592
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Transcriptional reporters were expressed in neurons and body wall muscle and were similarly expressed in both males and hermaphrodites. Colocalization with other reporters and anatomical criteria enabled identification of the expressing neurons as the ciliated sensory neurons OLL, PHA and PQR, the nonciliated sensory neurons URY and URX, the touch receptor neurons ALM, PLM, AVM and PVM, the interneurons in the retro-vesicular ganglion RIF and AVF, the command interneurons AVD and PVC, the ring motor neurons RMED and RMEV, and two other neurons tentatively identified as either PVQ or PVW and DB2. No expression was observed in amphid or male-specific neurons. |
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In snf-11(ok156) mutants, anti-SNF-11 staining was completely absent, confirming the specificity of the staining. Picture: Fig 4. |
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Expr7836
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In healthy young adults, the anti-SNF-11 antibody strongly stained the four RME neurons (RMED, RMEV, RMEL, and RMER). Faint staining of three additional GABAergic neurons (AVL, DVB, and RIS) was sometimes observed. Several non-GABAergic neurons, including RID, also seemed to stain. The ventral nerve cord DD and VD inhibitory motor neurons did not stain. Faint staining of the body wall, anal, and uterine muscles with the anti-SNF-11 antibody was observed in some animals. |
Staining of both the processes and the soma of each neuron were observed. In RMED and RMEV, a punctate staining pattern was observed in the posteriorly directed processes, possibly corresponding to synapses. |
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Picture: Figure 5 and Table 1. |
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Expr7837
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These Psnf-11::GFP fusions are expressed in the same neurons (RMEs, AVL, DVB, RIS, and RID) that stained with the anti-SNF-11 polyclonal antibodies. Expression was also noted in two additional neurons near the pharynx as well as two neurons in the retrovesicular ganglion. There were no apparent differences in expression between the two reporters, suggesting that the 1.9-kb region is sufficient to drive expression in all snf-11 positive cells. In contrast to observations reported previously (Jiang et al., 2005 blue right-pointing triangle), authors did not observe snf-11 expression in the ventral cord inhibitory (DD and VD) motor neurons. However, they did observe robust expression in the body wall, anal, and uterine muscles that was not noted previously. In young animals, expression of the Psnf-11::GFP reporter in muscle cells is the most prominent aspect of the expression pattern. |
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Expr15182
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In wild type L1 larvae, ceh-5p::GFP showed robust expression in head muscles, a subset of head neurons (including the RME neurons), and five or six cells in the tail including the PVQL/R neurons. Weak ceh-5p::GFP expression was also seen in the coelomocytes and the pharyngeal terminal bulb. |
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Temporal description. |
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Expr11629
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Pcwn-2::mCherry signal first appeared in the intestine before the expression of Punc-25::GFP during embryogenesis. At late embryonic stage when RMED/V neurite start extending posteriorly, the highest expression of CWN-2 is found at posterior pharyngneal bulb and the pharyngeal-intestinal valve. When animals are at L1 stage, the RMED/V neurites grow towards the posterior pharynx, where CWN-2 displays relatively higher expression level. The expression pattern of CWN-2 at both embryonic and L1 stages correlates well with the attractant role of CWN-2 during neurite outgrowth. After L1 stage, the neurites continue their growth and pass the posterior pharynx; meanwhile CWN-2 is also found expressed in body muscles and some ventral cord neurons. |
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avr-15 = GluClalpha2 |
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Expr1454
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The transformed animals exhibited fluorescence in all of the muscles of the metacorpus (pm4) and the isthmus (pm5), precisely those muscles onto which M3 synapses. The GFP staining of pharyngeal muscle began shortly before hatching and persisted throughout adulthood. Strong staining was also seen in a few neurons of the head, including RMED, RMEV and the bilaterally symmetric RMGs. Weak staining was seen in unidentified ventrally located neurons contributing to the dorsal and ventral sublateral nerve cords. Two ventral cord neurons near the anus stain consistently, namely DA9 and a more anteriorly located neuron that is likely to be VA12. |
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Picture: Fig. 2a, b. |
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Expr8733
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MAGI-1::GFP was expressed in several interneurons, including AVA, AVD, AVE, RIM, and RIA. |
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Since these gfp fusions lack the introns and the 3' untranslated region, they might be lacking potential regulatory sequences. In that case, the gfp expression patterns may not precisely represent those of the endogenous kin-8 gene. cam-1 is called kin-8 in this article. |
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Expr2267
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Expressed in chemosensory neurons in amphid: ASH. Other sensory neurons: ADE, FLP. Touch receptor neurons: AVM, ALM, PVM, PLM. Amphid interneurons: AIY, AIZ. Other interneurons: RIC, RMG, RIS, DVA, AVA, AVE, PVC, AVK, PVQ. Interneurons?: ALN, BDU, SDQ. Ring motor/inter neurons: RMD, RMDV. Ring motor neurons: RMED, RMEV Five neurons out of the following six, RIV, AVH, AVB, AVJ, AVD, AIN. About seven neurons in retrovesicular ganglion. Pharyngeal muscles in procorpus and isthmus. M4 and several pharyngeal neurons. A part of intestine and a few body wall muscles near the head (weak). Distal tip cells (sometimes and weak). A few ventral motor neurons and seam cells (rarely and weak). The expression patterns did not appear to change through the larva to adult stages. Embryonic expression was also observed. |
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Picture: Fig 3. |
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Expr8694
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Expression in the alimentary canal: Strong and consistent expression in M5, I1, I3, I6, NSM. Weak or rare expression in posterior arcades. Expression in the nervous system: Phsh, ADA, ADE, ADL, AIN, AIY, ALM, AUA, AVA, AVD, AVH, AVJ, AVK, AVM, AWB, BDU, CAN, CEP, DAn, DBn, DDn, DVB, DVC, FLP, HSN, IL1, IL2, LUA, OLL, PDA, PDB, PDE, PHA, PHB, PHC, PLM, PLN, PVC, PVD, PVM, PVN, PVP, PVQ, PVR, PVT, PVW, RIB, RIC, RIF, RIP, RIS, RME, SDQ, SIA (early larva), SIB (early larva), SMB (early larva), SMD (early larva), URA, URB, VAn, VBn, VCn, VDn, M5, I1, I6, NSM. Expression in the reproductive system: In adult stage, expressed in vulval muscle, uterine muscle, HSN, VCn. In developing larva stage, expressed in HSN, VCn, and anchor cell. |
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