1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003210 | mel-28 | C38D4.3 | Caenorhabditis elegans |
| Primary Identifier | Expr4321 | Remark | Visualization of GFP::MEL-28 in living embryos and immunofluorescence labeling show the same localization patterns, which are abolished in mel-28(RNAi) animals. |
| Subcellular Localization | MEL-28 shuttles dynamically between the nuclear periphery and the kinetochore during the cell cycles of early embryogenesis. During meiosis, MEL-28 is present on the nuclear periphery in the oocyte, on the condensing oocyte chromosomes, and on meiotic chromosomes (polar bodies) in the fertilized embryo. During interphase of the first mitotic cycle, MEL-28 is present at the nuclear periphery and as puncta within the nucleus. On the nuclear periphery, MEL-28 localization overlaps with the interior rim of nuclear-envelope marker mAB414 (which in C. elegans recognizes primarily nucleoporins NPP-9 [Nup358] and NPP-10 [Nup98/96]. As the pronuclei meet, each nucleus shows a dynamic reorganization of the internal puncta. By metaphase, the scattered dots resolve into pairs of stripes on either side of the metaphase plate, a pattern indicative of holocentric kinetochore localization. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003210 | mel-28 | C38D4.3 | Caenorhabditis elegans |
