WormMine: AnatomyTerm WBbt:0004010

• Definition: Post-embryonic cell of pedigree V6L.pppppapp, male sensory rays, structural cell.
• Name: R6stL
• Primary Identifier: WBbt:0004010
• Synonym: lineage name: V6L.pppppapp

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree V6L.pppppapp	V6L.pppppapp nucleus		WBbt:0002681

0 Expression Clusters

24 Expression Patterns

• Primary Identifier: Expr4679
• Remark: sin-3 = pqn-28 according to this paper.
• Pattern: When the sin-3 expression profile was examined in transgenic animals using a gfp reporter driven by a 1.5 kb sin-3 5'-flanking sequence, the sin-3::gfp signal was detected in all the ray structural cells. In addition, sin-3::gfp expression was observed in the inner labial neurons, socket cells, the cephalic neurons in the head region and the ventral nerve cord from L1 to adult stage.

• Primary Identifier: Expr2548
• Remark: Most of the tissues expressing rcn-1 overlap with those observed in calcineurin GFP and by immunostaining, including lateral hypodermal cells, vulva muscle tissue, nerve cords, and diverse neuronal expression. Reporter gene fusion type not specified.
• Pattern: GFP analysis of the transformed animals revealed that rcn-1 is expressed from late embryonic stages to adulthood in diverse tissues, including lateral hypodermal cells, marginal cells of the pharynx, vulva epithelial cells, ventral and dorsal nerve cords and commissures, and various neurons in the anterior and posterior portions of the worm. Male C. elegans displayed rcn-1 expression in male tail structures including the diagonal muscles, sensory rays, and spicules. GFP expression analysis of a shorter 1.6 kb 50 upstream fragment of rcn-1 revealed vulva muscle expression in addition to the aforementioned expression patterns.

• Primary Identifier: Expr1051
• Pattern: The unc-130::GFP construct is expressed in a dynamic pattern during embryogenesis in head hypodermal cells, as well as in muscle and intestinal precursor cells. In adults, unc-130::GFP is observed in ventral muscle, as well as in intestine and other cells in the head and tail. In adult males, unc-130 is also expressed in the structural cells and two neurons of each ray.

• Primary Identifier: Expr1118
• Pattern: The tissue distribution of HSP43 is localized to specific cells of the vulva, to the spermathecal valve and junctions between cells of the spermathecal cage. It is also concentrated in regions of contact between muscle cells or between muscle cells and the overlying hypodermis. In males, HSP43 was also found to be concentrated in specialized structures of the tail, including rays, copulatory spicules and other structures too poorly resolved to permit reliable identification. This signal was HSP43-specific, and not due to autofluorescence of tail structures, since it was abolished when the antibody was pre-incubated with excess HSP43 protein.

• Primary Identifier: Expr2938
• Pattern: GFP was observed from the embryo stage through to the adult stages. In the adult animal it was expressed in the pharynx, intestine, body wall muscle, gonad, distal tip cells and/or germ cells, nervous system, male tail rays, and spicule. chn-1 is probably expressed in all tissues.

• Primary Identifier: Expr1837
• Pattern: Expression of eff-1p::gfp was silent through the first third of embryogenesis, first appearing about 230 min after first cleavage in a subset of epidermal precursor cells. Over the next 3 hr, these and additional fluorescent cells were observed to migrate over the ventral and dorsal surfaces of the embryo, and the majority of GFP-positive cells fused to form the hyp6 and hyp7 syncytia. As elongation progressed, GFP was also expressed in a pair of cells that fused to form the binucleate "tail spike" . After hatching, eff-1p::gfp expression persisted in large epidermal syncytia through adulthood. Mononucleated epidermal cells-including the seam cells and the VPCs-remained nonfluorescent until shortly before undergoing larval fusion events. More specifically, GFP was seen in (1) nonstem daughters of the seam cells shortly before they fused into hyp7; (2) vulval cells invaginating to form toroids during morphogenesis; and (3) the rays and fan of the adult male tail. Expression was also seen in nonepidermal organs known to contain syncytia, including the pharynx and uterus. Interestingly, a few cells that express eff-1p::gfp have never been observed to fuse, such as some ventral epidermal precursors in the embryo and several neurons.

• Primary Identifier: Expr7804
• Remark: Picture: Fig 3D to 3R. To define the specific cellular requirement, the onset of mab-7 expression in these cells was correlated with the first appearance of ray swelling in mab-7 mutant males. Ray abnormality first appeared when ray retraction started, which was well before mab-7::gfp expression in the structural cells was detectable prior to the final molting. The hypodermal expression profile of mab-7 at the late L4 stage, however, matched perfectly with the timing of this ray morphogenesis.
• Pattern: Transgenic animals carrying these reporters displayed identical GFP signals in their hypodermis, structural cells, vulva, PQR, body seams and several neuronal processes in the head region at different developmental stages. The onset of mab-7::gfp expression was first detected in the hypodermis at the 2-fold stage. This hypodermal signal stayed on throughout the larval stages until the animals entered their adulthood. However, GFP expression in the body seam appeared only after the L4 stage and was maintained in adults. In the male tail, a GFP signal was detected at the late L4 stage in the structural cells when the ray retraction process was almost complete but prior to molting. This structural cell expression was also maintained in adults.

• Primary Identifier: Expr1628
• Pattern: In wild-type animals, the mab-21::gfp fluorescent signal was first observed in embryos at the beginning of gastrulation. The expressing cells were dorsal hyp 7 cells before their fusion. From this stage on, the signal could be detected in other larval and adult animals in tissues including hypodermal cells, a group of neuronal cells near the pharyngeal bulbs, neurons along the ventral nerve cord, some body wall muscles and the ray cells. Neuronal cells around the anal ganglia region identified as HOA, HOB in the males as well as PLM, PQR, and PVC. In addition to two unidentified anterior neurons, AVM and VA3 were also confirmed to express mab-21::gfp. The mab-21::gfp fusion transgene was expressed in neuron A of all rays, neuron B of rays 3 and 6 and more importantly, the structural cell of only ray 6.

• Primary Identifier: Expr1885
• Pattern: In animals transgenic for the smp-1::lacZ reporter constructs, beta-galactosidase is first detected in epidermal cells at the beginning of morphogenesis 200 minutes after fertilization. GFP fluorescence from smp-1::gfp expression is initially observed at approximately the 50 cell stage in the E lineage. Later, in larvae and adults, GFP can be seen in all body wall, vulval, uterine and enteric muscles, as well as male-specific muscles of the tail and copulatory system. In adults, smp-1::gfp is expressed in the tail tip (hyp 10), in ray 6 and in the spicules of the adult male. Approximately 10 sensory neuron support cells in the head with dendrites extending to the tip of the head, also express the smp-1 GFP and beta-galactosidase transcriptional reporters. GFP fluorescence is observed in several individual cells, including an interneuron (tentatively identified as AVL), the excretory channel, the distal tip cells (DTCs) throughout their migration, somatic cells of the gonad, and epidermal cells hyp 4 (and possibly hyp 3) and hyp 10. In the adult, expression was observed in the fused seam cell syncytium comprising the lateral epidermis. Although ray 6 expresses in the adult male tail, it is difficult to determine whether the ray 6 precursors or any other ray or SET precursors or progeny express smp-1::gfp during the L3 and L4 stages of development when the male tail is forming. This is because GFP fluorescence in the male sex-specific muscles is so bright at this stage as to obscure what may be faint expression of other cells in the male tail.

• Primary Identifier: Expr2951
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2952
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2953
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2954
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2955
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2956
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2957
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2958
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2959
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2960
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2961
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2962
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr2963
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in male sensory rays.

• Primary Identifier: Expr943
• Remark: The same pattern of expression was also noted when a genomic fragment spanning nucleotides I-1630 was used to drive the GFP reporter.
• Pattern: Both hermaphrodites and males expressed GFP in a group of cells around the pharyngeal corpus, which represented either the sheath or socket cells (PDEso) in the tail, as defined by their openings, position and morphology. GFP expression in these sensory support cells was detected soon after their birth and was maintained into adulthood. A GFP signal was detected in all the ray structure cells of the male tail, but not in the hypodermal tissue. Expression in nine pairs of structure cells began just prior to the ray retraction period, peaked during the retraction and declined upon completion of ray extension.

• Primary Identifier: Expr2929
• Reporter Gene: Transgenic animals carrying an integrated array of the transcriptional reporter evIs136[plx-2::gfp] or the translational reporter evIs168[plx-2::GFP] were analyzed. The transcriptional and translational plx-2 GFP reporters are expressed in the same cells including several that undergo guided migrations during development.
• Pattern: plx-2 expression occurs predominantly in the nervous system from embryonic to adult stages. The onset of plx-2 reporter expression coincides with the normal timing of outgrowth or migration (e.g., CAN) of these neurons. Several nonneuronal tissues also express evIs136[plx-2::gfp] and evIs168[plx-2(+)::GFP] including gonadal sheath, vulva precursors, and excretory canal. Of most relevance, expression of both the transcriptional and translational reporters occurs throughout a number of stages of sensory ray morphogenesis in the male tail. Expression of the plx-2 translational reporter evIs168[plx-2::GFP] is observed during ray morphogenesis at the Rn stage in the early L3 larva in R1, 3, 5, and 7. This reporter continues to express faintly in the Rn.a descendents that form rays 1, 3, 5, and 7 of late L3 larvae, which coincides with the period during which lineally related Rn.a descendants sort from neighboring Rn.a descendants to form distinct rays. However, in mid-L4 larvae, expression appears restricted to the ray structural cell (Rnst) for rays 3, 5, 6, and 7, and this expression continues into the late-L4 larval stage. Ray 1 expression of both reporters is undetectable in late L4 larvae.

0 Life Stages

4 Parents

Definition	Name	Synonym	Primary Identifier
hypodermal support cell that form a tube surrounding ray neuron dendrites.	ray structural cell	Rnst	WBbt:0008276
a cell that is born in a worm after hatching.	post-embryonic cell		WBbt:0007030
male sensory organ, sixth from most anterior, left.	ray 6 left		WBbt:0006961
post-embryonic cell of pedigree V6L.ppppaap	V6L.ppppaap		WBbt:0007514