WormMine: ExpressionPattern Expr16428

• Pattern: Transgenic worms expressing mCherry under the daf-42 promoter showed expression in seam cells between 48 and 60 HAE, which corresponds to the time of appearance of the dead L2d phenotype. At 60 HAE, we observed expression in the seam cell, hypodermis, and at the surface of the worm. At 72 HAE, ecdysis had begun, and some worms had detached their head or tail from the old L2d cuticle, and the DAF-42::GFP signal did not remain in the L2d cuticle but was localized with the dauer worm inside the L2d cuticle. Together, our results show that daf-42 is expressed during the L2d-to-dauer transition in seam cells and is secreted toward the surface of the dauer worm, such as the hypodermis or cuticle.
• Primary Identifier: Expr16428

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
a rigid external coating which is secreted by the hypodermis, seam and some transitional epithelial cells which covers the outer body, the major openings into the body cavity from the exterior, and two large sensory bristles, the male spicules. The cuticle of the spicules, the hook, and of some portions of the spicule channels is especially rigid and is probably sclerotized. Similar sclerotic features are occasionally noted in the ventral surface of the male tail fan.	cuticle	exoskeleton	WBbt:0005755

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00021496	daf-42	Y40C5A.3	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	A third stage larva specialized for dispersal and long term survival.		dauer larva Ce	WBls:0000032
	The stage during L2d-dauer molt, after the brief lethargus period, when an animal sheds off old cuticle.		L2d-dauer ecdysis Ce	WBls:0000048