WormMine: ExpressionPattern Expr11526

• Pattern: CYP33E2 promoter-driven expression of GFP occurred exclusively in the pharynx and, not visible in each individual, in the pharyngeal-intestinal valve of the nematodes. This type of strong pharynx-restricted expression was observed throughout larval development and in adult nematodes. Expression was most prominent in the pharyngeal pro- and meta-corpus. Confocal imaging suggested marginal, muscle, and/or epithelial cells as the major expression sites of the pCYP33E2::GFP construct within the pharynx. Radially, only marginal cell types are continuously organized with three-fold symmetry around the pharyngeal lumen. Imaginary cross sections derived from confocal imaging series of the pro- and meta-corpus indicated that the GFP reporter was expressed in the three marginal mc1 cells, but not in the pm3 and pm4 muscle cells. A further labelling of the mc2 and mc3 marginal cells in the isthmus and terminal bulb becomes then visible. Expression was observed in finger-like fluorescent structures that represent the interlocking extensions that hold marginal cells to muscles. Furthermore, an expression of pCYP33E2::GFP also in the epithelial e1, e2, and e3 cells seems most likely. Fluorescence might also correspond to pm2 muscle cells.
• Primary Identifier: Expr11526

16 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A group of six equivalent cells forms a tightly constructed `valve` that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine.	pharyngeal-intestinal valve	cardia	WBbt:0005767
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
cell that makes up epithelium in pharynx.	pharyngeal epithelial cell	pharyngeal epithelium	WBbt:0005459
posterior segment of pharyngeal corpus.	metacorpus		WBbt:0003711
anterior section of pharyngeal corpus.	procorpus		WBbt:0003713
a type of pharyngeal cell that connects basement membrane to the apices of triangle-shaped pharyngeal lumen.	marginal cell		WBbt:0003673
marginal cell, type 3.	mc3		WBbt:0003707
marginal cell, type 2.	mc2		WBbt:0003708
second layer pharyngeal muscle cell.	pm2	m2	WBbt:0003742
pharyngeal epithelial cell, type 2.	e2		WBbt:0003716
pharyngeal epithelial cell, type 1.	e1		WBbt:0003718
pharyngeal epithelial cell, type 3.	e3		WBbt:0003714
Pharyngeal marginal cell	mc1V	lineage name: ABalpappppa	WBbt:0004299
Pharyngeal marginal cell	mc1DL	lineage name: ABalpaapapa	WBbt:0004301
Pharyngeal marginal cell	mc1DR	lineage name: ABaraaapapa	WBbt:0004300

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00018334	cyp-33E2	F42A9.5	Caenorhabditis elegans

0 Life Stages