WormMine: ExpressionPattern Expr2286

• Pattern: Indirect immunofluorescence with EU102, demonstrates expression of the two largest isoforms in all muscles except for the pharynx. The pattern of expression and localization of Ce titin was examined in embryonic and larval muscles. During the 1.5 and 2-fold stages no specific staining was observed. Then, first appearing during the 2.5-fold stage, Ce titin appears in puncta which seem to follow the myofibril tracts. This punctate staining persists in the 3-fold stage, but then staining all but disappears in L1 larvae. However, during the L2 or L3 stages, and continuing during the remainder of development, Ce titin staining is localized in the same dashed pattern as seen in adult muscle.
• Primary Identifier: Expr2286
• Remark: Because the N-terminal 96 amino acid residues fused to GFP localizes to dense bodies, and because a major component of dense bodies is a-actinin, the localization of EU102 epitopes was determined in an alpha-actinin null mutant background. These animals, with the genotype atn-1(ok84), have normal motility and nearly normal muscle structure by polarized light microscopy. By EM, however, they have extra accumulations of actin near the ends of the muscle cells and, significantly, the dense bodies are shorter and more broadly based than normal. Staining of atn-1(ok84) muscle with EU102 is in a much broader pattern than wild-type and there is also loss of the "dashed" appearance. It is possible that in an alpha-actinin null background, the largest Ce titin isoforms are not properly anchored to the dense bodies. Sequence: Y38B5A.
• Subcellular Localization: As visualized with confocal immunofluorescence microscopy, EU102 stains the center of I-bands, between adjacent dense bodies. This was determined in three ways. When co-stained with antibodies to alpha-actinin, a major component of nematode dense bodies, EU102 staining appears to lie between, and does not overlap, the dense bodies. When co-stained with antibodies to actin, which gives a ladder-like pattern, EU102 appears to lie within the "rungs of the ladder". Finally, co-staining with antibodies to tropomyosin, that has been shown to localize to the outer portions of the I-bands in a linear pattern, showed that EU102 labels between the rows of tropomyosin staining. EU134 localizes to the I-band, but with a broader distribution than that obtained with EU102 antibodies. EU134 stains an area between dense bodies (overlapping with EU102 staining), as well as an area deeper into the I-band.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006436	ttn-1	W06H8.8	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019