WormMine: ExpressionPattern Expr2883

• Pattern: SUR-7HGFP was expressed in most tissues with the notable exception of intestinal cells. Under a UV-light dissecting microscope, GFP was seen to be strongly expressed in the pharynx, some tail cells and the vulva. Under higher magnification, GFP was observed in many if not all neurons, muscles and hypodermal cells.
• Primary Identifier: Expr2883
• Reporter Gene: The sur-7HGFP construct is functional since the expression of this construct rescues sur-7(ku119);let-60(n1046).
• Subcellular Localization: SUR-7HGFP was not localized to the plasma membrane, as was the case for a CDF-1HGFP construct, but was enriched at the nuclear periphery and throughout the cytoplasm in a fenestrated pattern. This localization is endoplasmic reticular for two reasons. First, this expression pattern is indistinguishable from that of a C. elegans YFPHTRAM construct (TRAM is an endoplosmic recticulum (ER) transmembrane protein). Secondly, several CDF family members are known to be ER localized.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675
posterior region, from rectum to the end	tail		WBbt:0005741

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006353	sur-7	F01G12.2	Caenorhabditis elegans

0 Life Stages