WormMine: ExpressionPattern Expr12508

• Pattern: TSP-17::GFP fusion protein expression was observed in all dopaminergic neurons: it was uniform along axons and dendrites of both dorsal and ventral pairs of CEP neurons, as well as in ADE neurons and in the posterior PDE neurons. Within the cell body, the TSP-17::GFP fusion seems to be excluded from the nucleus, a pattern that is more evident in a 'close-up' image of a PDE neuron, where the signal appears to form a ring-like structure around the nucleus. mCherry aggregates (coming from the pdat- 1::mcherry::let858 39UTR construct, which are not linked to neurodegeneration) form dot-like structures in dendrites and axons (arrows), and the surrounding TSP-17 fluorescent signal suggests plasma membrane expression. TSP- 17 enrichment at the plasma membrane can be observed most prominently in the large cells of the vulva and the sheath cells enclosing the spermatheca. In the spermatheca, TSP-17::GFP expression is also clearly enriched around the nucleus, possibly localizing to the nuclear membrane or endoplasmic reticulum. TSP-17::GFP is also expressed in multiple neurons throughout worm development. For instance, the NSM serotonergic neuron, which is characterized by extensive axon sprouting, shows TSP-17::GFP expression along its entire length. Prominent expression was also observed in the muscles of early stage larvae. Finally expression also appears to be apparent in muscles of the adult head. In summary, the TSP-17::GFP expression indicates that TSP-17 is expressed in dopaminergic neurons.
• Primary Identifier: Expr12508

16 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
body wall muscle in the head, including first eight cells of each muscle quadrants which are innervated by nerve ring neurons.	head muscle	head body wall muscle	WBbt:0006761
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679
Neuron, dopaminergic of postderid sensillum	PDEL	lineage name: V5L.paaa	WBbt:0004383
Neuron, dopaminergic of postderid sensillum	PDER	lineage name: V5R.paaa	WBbt:0004382
Pharyngeal neurosecretory motorneuron, contain serotonin	NSML	lineage name: ABaraapapaav	WBbt:0004457
Pharyngeal neurosecretory motorneuron, contain serotonin	NSMR	lineage name: ABaraapppaav	WBbt:0004446
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828
Anterior deirid, sensory neuron, dopaminergic	ADEL	lineage name: ABplapaaaapa	WBbt:0004009
Anterior deirid, sensory neuron, dopaminergic	ADER	lineage name: ABprapaaaapa	WBbt:0004007
Cephalic neurons, contain dopamine	CEPVL	lineage name: ABplpaappppa	WBbt:0004933
Cephalic neurons, contain dopamine	CEPVR	lineage name: ABprpaappppa	WBbt:0004931
Cephalic neurons, contain dopamine	CEPDL	lineage name: ABplaaaaappa	WBbt:0004938
Cephalic neurons, contain dopamine	CEPDR	lineage name: ABarpapaappa	WBbt:0004937

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006643	tsp-17	C02F12.1	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041