WormMine: ExpressionPattern Expr7858

• Pattern: CeVPS-27 was detected ubiquitously, as early as in the 2-cell embryo and persisted throughout the embryonic and larval development and in adult animals. At mid-embryogenesis, when epithelialization proceeds, CeVPS-27-positive vesicles were preferentially enriched in hypodermal, pharyngeal and intestinal cells. The specificity of the signal was confirmed by its complete disappearance after inactivation of Cevps-27 by RNAi. During larval development and in adults, CeVPS-27 was expressed ubiquitously but remained more abundant in the intestine, the hypodermis, the pharynx and in the adult epithelial tissues; namely the uterus, the vulva, the spermatheca and the gonad. CeVPS-27 could also be found in the excretory canal, and the six scavenger cells called coelomocytes.
• Primary Identifier: Expr7858
• Remark: Cevps-27 = C07G1.5 Immunohistochemical analyses with anti-CeVPS-27 antibodies (Figure 2AD,GJ) and observation of CeVPS-27::GFP transgenic worms (Figure 2E,F,K) revealed a similar expression pattern. Picture: Figure 2, Figure 3. Several arguments support the idea that CeVPS-27 localizes to endosomes. First, CeVPS-27-positive vesicles are not lysosomes as revealed by an absence of colocalization of CeVPS-27::GFP with the lysosomal marker lysotracker. Second, CeVPS-27 is localized in vesicles close to the plasma membrane in epithelial cells and in the coelomocytes, which are specialized in endocytosis of fluid and macromolecules from the body cavity. Finally, the inactivation of the homolog of the class E vps-23, which in yeast and mammals results in the formation of enlarged endosomes, induced the production of enlarged CeVPS-27-positive vesicles. Together, these data indicate that CeVPS-27 is localized at the endosomal membrane in C. elegans.
• Subcellular Localization: The protein was detected as a punctate pattern in the cytoplasm indicating a vesicular localization. At mid-embryogenesis, when epithelialization proceeds, CeVPS-27-positive vesicles were preferentially enriched in hypodermal, pharyngeal and intestinal cells, where they localized close to the apical membrane as revealed by the apical junction marker AJM-1. Later during morphogenesis, CeVPS-27-positive vesicles became more uniformly localized. Several arguments support the idea that CeVPS-27 localizes to endosomes (see Remark).

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
female genital.	vulva		WBbt:0006748
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775
organ in hermaphrodite animal that produces both ova and sperm.	hermaphrodite gonad	ovotestis	WBbt:0005178

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004101	hgrs-1	C07G1.5	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003