WormMine: ExpressionPattern Expr8581

• Pattern: The expression of genomic magi-1::gfp and magi-1::rfp chimeric transgenes, which contain 4 kb of upstream sequence and either the complete magi-1 ORF or only the genomic magi-1 long isoform sequences, yielded similar results The magi-1L:::gfp reporter transgene reflects the expression of the long isoform. Expression of MAGI-1L::GFP was detected in the pharynx, head neurons, ventral cord, and vulva of animals mosaic for the extrachromosomal array. Expression of MAGI-1L::RFP was also detected in the pharynx, intestine, vulval epithelia (L4 stage), and vulval muscle (adult stage) of animals mosaic for the extrachromosomal array. This antibody labeled both neurons and epithelial junctions in immunofluorescence experiments. Within the hypodermis and intestine, there was a high degree of colocalization between MAGI-1 and DLG-1. The anus and vulva also were labeled in larval and adult animals. Finally, MAGI-1 expression was observed in some neurons in the head, including a subset of the GLR-1::GFP-expressing command interneurons. MAGI-1 is expressed in the backward but not the forward command interneurons; MAGI-1 expression was not detected in any of the mechanosensory neurons. MAGI-1 was detected in the neurons AVA, AVD, and SMD, as well as RMD and RMDV.
• Primary Identifier: Expr8581
• Remark: Picture: Figure 2, Fig. S2BE.
• Subcellular Localization: Anti-MAGI-1 antibody detected MAGI-1 localization to puncta along the ventral cord; however, MAGI-1 is expressed in more neurons than just those that express GLR-1, making analysis of colocalization in the ventral cord difficult. To help restrict the analysis to the GLR-1-expressing neurons, both MAGI-1 isoforms were expressed as mRFP chimeric proteins under the glr-1 promoter; both substantially colocalized with GLR-1.

16 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
female genital.	vulva		WBbt:0006748
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Ring motor neuron/interneuron, has a posteriorly direct ed process that runs sublaterally	SMDVR	lineage name: ABarappppaa	WBbt:0004969
Ring motor neuron/interneuron, has a posteriorly direct ed process that runs sublaterally	SMDDL	lineage name: ABplpapaaaa	WBbt:0004972
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAL	lineage name: ABalppaaapa	WBbt:0003870
Ventral cord interneuron, synapses onto VA, DA, and AS motor neurons; formerly called alpha	AVAR	lineage name: ABalaappapa	WBbt:0003869
openning of the rectum	anus	anus interface	WBbt:0005364
Ring motor neuron/interneuron, many synapses	RMDL	lineage name: ABalpppapad	WBbt:0005037
Ring motor neuron/interneuron, many synapses	RMDR	lineage name: ABpraaaapad	WBbt:0005033
Ring motor neuron/interneuron, many synapses	RMDVL	lineage name: ABalppapaaa	WBbt:0005031
Ventral cord interneuron, synapses onto VA, DA, AS motorneurons; formerly called delta.	AVDL	lineage name: ABalaaapalr	WBbt:0003866
Ventral cord interneuron, synapses onto VA, DA, AS motorneurons; formerly called delta.	AVDR	lineage name: ABalaaapprl	WBbt:0003865
Ring motor neuron/interneuron, many synapses	RMDVR	lineage name: ABarapppaaa	WBbt:0005029

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00010444	magi-1	K01A6.2	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035