WormMine: ExpressionPattern Expr9107

• Pattern: To understand EGRH-1 function in regulating oocyte development, authors examined egrh-1::gfp expression and endogenous EGRH-1 expression using an antibody targeting a unique region at the EGRH-1 N-terminus in dissected adult hermaphrodites. Using both techniques, EGRH-1 expression was observed in the sheath cells and distal tip cells of the somatic gonad, as well as in the intestine and sperm. egrh-1::gfp was expressed in embryos through adult stages in most somatic tissues, including the gut, pharynx, body wall muscle, and nervous system.
• Primary Identifier: Expr9107
• Remark: Picture: Fig. 5. To test if EGRH-1 antibody staining was specific, authors stained egrh-1(tm1736) mutants, which are predicted to encode a truncated protein that would be recognized by our antibody, and egrh-1(tm1736) with egrh-1 expression further reduced by RNAi. Staining was decreased in both cases, indicating that this antibody stain is specific for EGRH-1.
• Subcellular Localization: EGRH-1 was localized to nuclei in all tissues except sperm, where it appeared perinuclear. Importantly, no egrh-1::gfp expression or EGRH-1 antibody staining was observed in oocytes.

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828
Male haploid germ cell.	sperm	spermatozoa	WBbt:0006798

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007772	egrh-1	C27C12.2	Caenorhabditis elegans

0 Life Stages