WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Anatomy Term :

Definition  organ in hermaphrodite animal that produces both ova and sperm. Name  hermaphrodite gonad
Primary Identifier  WBbt:0005178 Synonym  ovotestis

7 Children

Definition Name Synonym Primary Identifier
A valve tissue that connects the spermatheca and the uterus of a hermaphrodite gonad. spermathecal-uterine junction sp-ut valve WBbt:0006756
an accordion-like tube that contains sperm and is the site of oocyte fertilization. spermatheca   WBbt:0005319
The organ in which the eggs are developed and protected until laid. uterus   WBbt:0006760
Anterior lobe of the bi-lobbed hermaphrodite gonad. anterior gonad arm   WBbt:0005374
Posterior lobe of the bi-lobbed hermaphrodite gonad. posterior gonad arm   WBbt:0005466
immature gonadal structure of hermaphrodite. developing hermaphrodite gonad   WBbt:0006903
somatic (not germline) cell of the hermaphrodite gonad. hermaphrodite somatic gonadal cell   WBbt:0007815

4 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Genes upregulated in mes-4 relative to wild type. Confidence levels were determined using a two-tailed paired t-test. WBPaper00028483:Up_mes-4_vs_WT
  Genes upregulated in deps-1(bn124) relative to wild type. Confidence levels were determined using a z test performed in Excel followed by a correction for multiple testing in which the P-value was multiplied by 17,539 (the number of genes on the microarrays). After this correction, all genes with a fold change of >1.8-fold, P < 0.05 were selected for analysis. WBPaper00031477:Up_deps-1_vs_WT
  Genes downregulated in deps-1(bn124) relative to wild type. Confidence levels were determined using a z test performed in Excel followed by a correction for multiple testing in which the P-value was multiplied by 17,539 (the number of genes on the microarrays). After this correction, all genes with a fold change of >1.8-fold, P < 0.05 were selected for analysis. WBPaper00031477:Down_deps-1_vs_WT
  Genes downregulated in mes-4 relative to wild type. Confidence levels were determined using a two-tailed paired t-test. WBPaper00028483:Down_mes-4_vs_WT

12 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr12624 eel-1 mRNA appears enriched in the hermaphrodite gonad and is also detected at high levels in the early embryo. From Nematode Expression Pattern Database, http://nematode.lab.nig.ac.jp/  
    Expr11923 Consistent with the expression of the GFP reporter transgene, gcn-1 mRNA was observed in most somatic cells. In addition, gcn-1 mRNA was abundant in the germ cells in the hermaphrodite gonad.  
    Expr3298 Expressed in body wall muscle, hypodermis, intestine, neurons, gonad, pharyngeal muscle, spermatheca and vulva.  
Cevps-27 = C07G1.5 Immunohistochemical analyses with anti-CeVPS-27 antibodies (Figure 2AD,GJ) and observation of CeVPS-27::GFP transgenic worms (Figure 2E,F,K) revealed a similar expression pattern. Picture: Figure 2, Figure 3. Several arguments support the idea that CeVPS-27 localizes to endosomes. First, CeVPS-27-positive vesicles are not lysosomes as revealed by an absence of colocalization of CeVPS-27::GFP with the lysosomal marker lysotracker. Second, CeVPS-27 is localized in vesicles close to the plasma membrane in epithelial cells and in the coelomocytes, which are specialized in endocytosis of fluid and macromolecules from the body cavity. Finally, the inactivation of the homolog of the class E vps-23, which in yeast and mammals results in the formation of enlarged endosomes, induced the production of enlarged CeVPS-27-positive vesicles. Together, these data indicate that CeVPS-27 is localized at the endosomal membrane in C. elegans.   Expr7858 CeVPS-27 was detected ubiquitously, as early as in the 2-cell embryo and persisted throughout the embryonic and larval development and in adult animals. At mid-embryogenesis, when epithelialization proceeds, CeVPS-27-positive vesicles were preferentially enriched in hypodermal, pharyngeal and intestinal cells. The specificity of the signal was confirmed by its complete disappearance after inactivation of Cevps-27 by RNAi. During larval development and in adults, CeVPS-27 was expressed ubiquitously but remained more abundant in the intestine, the hypodermis, the pharynx and in the adult epithelial tissues; namely the uterus, the vulva, the spermatheca and the gonad. CeVPS-27 could also be found in the excretory canal, and the six scavenger cells called coelomocytes. The protein was detected as a punctate pattern in the cytoplasm indicating a vesicular localization. At mid-embryogenesis, when epithelialization proceeds, CeVPS-27-positive vesicles were preferentially enriched in hypodermal, pharyngeal and intestinal cells, where they localized close to the apical membrane as revealed by the apical junction marker AJM-1. Later during morphogenesis, CeVPS-27-positive vesicles became more uniformly localized. Several arguments support the idea that CeVPS-27 localizes to endosomes (see Remark).
No detailed description on expression patterns in other life stages.. Picture: Fig. 5.   Expr8275 GFP expression was observed in various tissues, including many neurons in the head and tail, head muscles, intestine, phasmid socket cells, spermatheca, and eggs. A weak expression was also observed in the cells of the gonad and vulva. The neurons expressing ser-3 promoter-driven GFP in the tail were identified as PHA, PHB, and PVQ neurons.  
    Expr1200002 Data from the TransgeneOme project  
    Expr1200323 Data from the TransgeneOme project  
    Expr3417   In mated females, extracellular MSP exhibits a graded distribution, with a sharp boundary between the -1 and -2 oocytes. Fluorescence intensity measurements indicate that MSP is localized in a graded manner from the spermatheca to the oocyte. Fluorescence intensity measurements also indicate that there is significant MSP staining over the -2 and -3 oocytes. To pinpoint the localization of MSP at the oocyte cell surface, a 3D confocal analysis of MSP localization was conducted a in mated females using the RME-2 yolk receptor to mark the oocyte plasma membrane and the early endosomal compartments. Three-dimensional image reconstructions of the data indicate that MSP localizes in three regions: (1) in superficial focal planes at the oocyte cell surface with RME-2 just beneath; (2) in the same plane as the RME-2 signal; and (3) within the oocyte beneath the plasma membrane. Using anti-MSP mAbTR-20 and visual inspection, 91% of mated female gonad arms exhibited extracellular MSP localization (n=36), with 39% showing extracellular MSP as far as the most proximal oocyte, and the rest exhibiting extracellular MSP only within the spermatheca. With confocal microscopy, extracellular MSP appeared both punctate and diffuse in the spermatheca, the gonad arm and the uterus. Analysis of 3D data stacks indicated that punctate extracellular MSP was enriched near spermatozoa on the spermathecal walls. The largest puncta were at the diffraction limit of the microscope (0.5 um) and were found nearby spermatozoa. In the proximal gonad arm, MSP was more diffuse and localized in focal plane slices near the oocyte surface. In the uterus, large MSP puncta was observed close to spermatozoa. Diffuse MSP was also observed in extracellular spaces surrounding embryos in the uterus. MSP puncta can be observed near spermatozoa in the uterus and spermatheca using wide-field microscopy, when these regions were less crowded with spermatozoa.
    Expr12286 exo-3 is abundantly expressed in the gonads of both hermaphrodites and males.  
    Expr12287 apn-1 is abundantly expressed in the gonads of both hermaphrodites and males.  
    Expr1200046 Data from the TransgeneOme project  
    Expr11924 Consistent with the expression of the GFP reporter transgene, abcf-3 mRNA was observed in most somatic cells. In addition, abcf-3 mRNA was abundant in the germ cells in the hermaphrodite gonad.  

0 Life Stages

2 Parents

Definition Name Synonym Primary Identifier
organ producing either sperm or ova. gonad   WBbt:0005175
cells or anatomical parts specific to the hermaphrodite sex hermaphrodite-specific anatomical entity   WBbt:0005758