WormMine: ExpressionPattern Expr13989

• Pattern: In the posterior pharyngeal bulb gfp::hum-7 is expressed throughout, including the pm6 and pm7 cells that help the grinder contract during feeding (Worm Atlas), while hmr-1::mKate2 is enriched only in apical regions, as expected. hmr-1::mKate2 is expressed all through the nerve ring axons, while gfp::hum-7 is expressed in adjacent cells, perhaps glia. Viewing this same strain on the surface shows that while hmr- 1::mKate2 is highly expressed in the seam cells, gfp::hum-7 shows no obvious overlap in the seam cells. A strain expressing RFP under control of the myosin heavy chain promoter, Pmyo-3::rfp (Viveiros et al., 2011), shares expression with gfp::hum-7 in body wall muscles. Therefore gfp::hum-7 appears to have broad expression, with enrichment in several types of muscle tissue including regions of the pharynx, and in body wall muscles. Expression is not overall enhanced in neurons, but may include support cells for neurons. Expression does not appear enhanced in epidermal cells, although there is precedent for epidermal signal to appear striped due to impingement from muscle or pharynx (Worm Atlas). Antibody staining with antibodies specific to body wall muscle support that at least some of the striped signals are in muscle.
• Primary Identifier: Expr13989

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
seventh pharyngeal muscle cell layer	pm7	m7	WBbt:0003721
cells that support sensory neurons, similar to glial cells in vertebrates. A category which collectively refers to socket cells, sheath cells, and structural cells, or their processes. All of these cells extend long processes which serve a supporting role, rather like glia, to form a protective environment around sensory neuron endings. In addition, some of these cells extend broad thin processes from their somata which wrap around neuronal ganglia, again in a glia-like fashion	accessory cell	support cell	WBbt:0005762
the last, posterior bulb of the pharynx.	terminal bulb		WBbt:0003732
sixth pharyngeal muscle cell layer	pm6	m6	WBbt:0003724

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002040	hum-7	F56A6.2	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023