WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  The expression pattern of MADD-2::GFP in animals carrying trIs31 was characterized in detail and is similar to the pattern observed in animals carrying trIs32. Weak MADD-2::GFP expression was first observed in the ventral blast cells during late gastrulation, followed by stronger expression in the overlying ventral hypodermal blast cells during enclosure. MADD-2::GFP expression can also be seen in myoblasts at the two-fold stage of development and persists in the BWMs throughout the life of the animal. From the three-fold stage of embryonic development, MADD-2::GFP is localized to the right side of the ventral hypodermal ridge and to the left side of the dorsal hypodermal ridge, where the ventral and dorsal major nerve cords, respectively, reside. MADD-2::GFP is also expressed in the vulval muscles, the anchor cell, the six ventral uterine precursor cells, the lateral seam cells, and the ray precursor cells and their descendents in males. Other ectodermal derivatives that express MADD-2::GFP include the hermaphrodite-specific neurons (HSNs), and some Q cell descendents, including the AVM, PVM, SDQr, and SDQl neurons. Primary Identifier  Expr9091
Remark  Picture: Fig 3, Fig S1, S2, S3. Subcellular Localization  Authors also expressed CFP-tagged MADD-2 in select body muscles by using the him-4 promoter. The him-4 promoter drives expression in the distal BWMs of each quadrant and not within the nervous system. In these transgenic animals, MADD-2::CFP is localized to the dense bodies and the muscle arm termini in a pattern that recapitulates MADD-2::GFP localization in strains carrying trIs31. Transgenic BWMs that are surrounded by nontransgenic cells show nearby localization of MADD-2::GFP at the hypodermal ridge, suggesting that MADD-2 is localized postsynaptically and not within axons. Within the BWMs, MADD-2::GFP is localized to the dense bodies that anchor thin fila ments to the extracellular matrix.

20 Anatomy Terms

Definition Name Synonym Primary Identifier
Epidermal layer. hypodermis epidermis WBbt:0005733
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
muscle associated with hermaphrodite vulva. vulval muscle   WBbt:0005821
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic HSNL lineage name: ABplapppappa WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic HSNR lineage name: ABprapppappa WBbt:0004757
Precursor of cells that form male sensory ray 4. R4   WBbt:0006935
Precursor of cells that form male sensory ray 3. R3   WBbt:0006934
Precursor of cells that form male sensory ray 2. R2   WBbt:0006933
Precursor of cells that form male sensory ray 1. R1   WBbt:0006932
Precursor of cells that form male sensory ray 8. R8   WBbt:0006939
Precursor of cells that form male sensory ray 7. R7   WBbt:0006938
Precursor of cells that form male sensory ray 6. R6   WBbt:0006937
Precursor of cells that form male sensory ray 5. R5   WBbt:0006936
Precursor of cells that form male sensory ray 9. R9   WBbt:0006940
anchor cell, induces vulva, part of hermaphrodite gonad. anchor cell AC WBbt:0004522
Neuron class of one neuron, posterior ventral microtuble cell, touch receptor. PVM lineage name: QL.paa WBbt:0004086
Neuron class of one sensory neuron, anterior ventral microtubule cell, touch receptor. AVM lineage name: QR.paa WBbt:0003832
Ant. lateral interneuron, process projects into ring SDQR lineage name: QR.pap WBbt:0004991
Posterior lateral interneuron, process projects into ring SDQL lineage name: QL.pap WBbt:0004993
Ventral Uterine precursor cell. ventral uterine precursor VU WBbt:0006780

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00016539 madd-2 C39F7.2 Caenorhabditis elegans

0 Life Stages