WormMine: ExpressionPattern Expr977

• Pattern: Neither immunoblotting nor immunostaining analysis with the anti-EAT-20 polyclonal antibody detected any signal in eat-20 mutants. At the 16-cell stage, patches of staining on the entire surface of embryos were first detected. From the comma stage on, staining was detected in coelomocytes, the nervous tissues, hypodermal cells, and the pharynx. At the comma stage, the apical surface of the alimentary canal was stained intensely and the basal surface of presumptive pharynx was moderately stained. The staining in the presumptive pharynx gradually weakened thereafter and disappeared completely at the late 3-fold stage. The staining of the apical surface of the pharynx and pharyngeal intestinal valve remained intense throughout the rest of the embryogenesis stage. Granular staining in the region surrounding the pharyngeal lumen was observed from the L2 stage on and spread to the external surface. Staining of the entire surface of the pharyngeal muscle was observed in the late L3 or L4 stage. The inner linings of the intestine and anus were intensely stained until the 2-fold stage, and then the staining became weak and disappeared completely at the 3-fold stage. The anterior-most intestinal cell became stained from the L3 stage. Staining was also observed in the nervous tissues. At the rostral end of the head, staining that seemed to correspond to the distal segments of labial process bundles was seen from the comma stage up to the adult stage. In larvae, the staining sometimes extended posteriorly to the level of the isthmus. A pair of cells, which might be support cells of sensory neurons posterior to the distal structures, were stained from the L1 stage up to the adult stage. The motor neurons in the ventral cord, which sometimes expressed GFP in the promoter trap line, were stained at the early L1 stage. This staining disappeared at the late L1 stage and, in the later stages, was replaced by segmental staining of the ventral nerve cord. The nerve ring and the nerve bundles that connect the nerve ring and the ventral nerve cord had dots of very faint staining. On the lateral body wall at the base of the tail spike, staining was detected from the L2 stage up to the adult stage, which may correspond to the axon of ALN neuron that expressed reporter GFP in the promoter trap lines. In the adult male tail, sensory rays were intensely stained. There was also extensive hypodermal staining. Weak staining of the seam cells began at the 3-fold stage and the staining became intense from the L3 stage up to the adult stage. Thin longitudinal bands were stained along the dorsal and ventral midline from the rostral end of the body to the base of the tail spike, which corresponds to the position of the dorsal and ventral hypodermal ridges. The hypodermal staining co-localizing with the position of the ventral nerve cord was the most intense. The hypodermal cells at the opening and inside of the vulva were stained from the L4 stage up to the adult stage. The hypodermis around the anus was stained from the L2 stage up to the adult stage. The coelomocytes were continuously stained from the comma stage up to the adult stage.
• Primary Identifier: Expr977

44 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
Post-embryonic cell of pedigree V6L.pppapaaa, male sensory rays, neuron, striated rootlet.	R4AL	lineage name: V6L.pppapaaa	WBbt:0004030
Post-embryonic cell of pedigree V6R.pppapaaa, male sensory rays, neuron, striated rootlet.	R4AR	lineage name: V6R.pppapaaa	WBbt:0004029
Post-embryonic cell of pedigree V6L.papapaaa, male sensory rays, neuron, striated rootlet.	R2AL	lineage name: R2L.aaa	WBbt:0004042
Post-embryonic cell of pedigree V6R.papapaaa, male sensory rays, neuron, striated rootlet.	R2AR	lineage name: R2R.aaa	WBbt:0004041
Post-embryonic cell of pedigree V6L.papppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R3BL	lineage name: V6L.papppapa	WBbt:0004034
Post-embryonic cell of pedigree V6R.papppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R3BR	lineage name: V6R.papppapa	WBbt:0004033
Post-embryonic cell of pedigree TL.appaaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R8BL	lineage name: TL.appaaapa	WBbt:0003990
Male sensory rays, neuron, darkly staining tip, open to outside	R8BR	lineage name: TR.appaaapa	WBbt:0003988
Post-embryonic cell of pedigree V6L.pppapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R4BL	lineage name: V6L.pppapapa	WBbt:0004028
Post-embryonic cell of pedigree V6R.pppapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R4BR	lineage name: V6R.pppapapa	WBbt:0004027
Post-embryonic cell of pedigree V6L.papapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R2BL	lineage name: V6L.papapapa	WBbt:0004040
Post-embryonic cell of pedigree V6R.papapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R2BR	lineage name: V6R.papapapa	WBbt:0004039
Post-embryonic cell of pedigree V5L.pppppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R1BL	lineage name: V5L.pppppapa	WBbt:0004048
Post-embryonic cell of pedigree V5R.pppppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R1BR	lineage name: V5R.pppppapa	WBbt:0004046
Post-embryonic cell of pedigree TL.appapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R9BL	lineage name: TL.appapapa	WBbt:0003968
Post-embryonic cell of pedigree TL.apappapa, male sensory rays, neuron, darkly staining tip, open to outside.	R7BL	lineage name: TL.apappapa	WBbt:0004001
Male sensory rays, neuron, darkly staining tip, open to outside	R7BR	lineage name: TR.apappapa	WBbt:0004000
Male sensory rays, neuron, darkly staining tip, open to outside	R9BR	lineage name: TR.appapapa	WBbt:0003966
Post-embryonic cell of pedigree V6L.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BL	lineage name: V6L.ppppaapa	WBbt:0004022
Post-embryonic cell of pedigree V6R.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BR	lineage name: V6R.ppppaapa	WBbt:0004021
sensillum of inner or outer labial.	labial sensillum		WBbt:0005107
Post-embryonic cell of pedigree TL.appaaaaa, male sensory rays, neuron, striated rootlet.	R8AL	lineage name: TL.appaaaaa	WBbt:0003994
Male sensory rays, neuron, striated rootlet	R8AR	lineage name: TR.appaaaaa	WBbt:0003992
Post-embryonic cell of pedigree V6L.ppppaaaa, male sensory rays, neuron, striated rootlet.	R5AL	R5L.aaa	WBbt:0004024
Post-embryonic cell of pedigree V6R.ppppaaaa, male sensory rays, neuron, striated rootlet.	R5AR	lineage name: V6R.ppppaaaa	WBbt:0004023
Post-embryonic cell of pedigree V6L.papppaaa, male sensory rays, neuron, striated rootlet.	R3AL	lineage name: R3L.aaa	WBbt:0004036

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001148	eat-20	H30A04.1	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002