WormMine: ExpressionPattern Expr1632

• Pattern: Although the antibody and reporter patterns coincide in a number of key aspects, a few differences are evident. Expression in the head hypodermal cells upon hatching is only faintly detected with the gfp reporter construct. Moreover, only a few neurons in the head express the ceh-32::gfp construct, compared with the immunostaining results. These differences could reflect that positive control elements are missing or inactive in the reporter transgenes. The expression of ceh-32 begins during the embryonic development, during the gastrulation stage (in 100-min-old embryos), and persists until adults. Embryonic expression of ceh-32 is detected in the anterior part of the embryos in head hypodermal and neuronal precursor cells. Upon hatching, ceh-32 is expressed in the hypodermal and neuronal cells of the head as well as in the somatic gonad. The head hypodermal nuclei expressing CEH-32 were identified as the nuclei of hyp3, hyp4, hyp5, and the first ventral nuclei of hyp6. In the head neurons, ceh-32 is found expressed in 12 cells of the anterior ganglion, in the sensory neurons ADL, in a pair of neurons in the dorsal side of lateral and ventral ganglion, and in 8 neurons in the ventral side of the lateral and ventral ganglion. Some weakly expressing cells were not identified. In the somatic gonad, the expression of ceh-32 begins at the L1/L2 stage in the sheath/spermatheca (SS) precursor cells, and this expression is maintained during the development of the somatic gonad in the gonadal sheath cells. In the adult worm, all the gonadal sheath cells express ceh-32.
• Primary Identifier: Expr1632
• Subcellular Localization: The CEH-32 protein is detected exclusively in the nuclei.

10 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Cylindrical hypodermal syncytium in head	hyp3	lineage name: ABplaapaaaa	WBbt:0004690
Cylindrical hypodermal syncytium in head	hyp4	lineage name: ABarpapapa	WBbt:0004687
Cylindrical hypodermal syncytium in head	hyp5	lineage name: ABarpappap	WBbt:0004685
Amphid neuron, prob. chemosensory	ADLL	lineage name: ABalppppaad	WBbt:0003997
Amphid neuron, prob. chemosensory	ADLR	lineage name: ABpraaapaad	WBbt:0003995
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828
Cylindrical hypodermal syncytium in head	hyp6	lineage name: ABarpaapaa	WBbt:0004679
The left and right lateral ganglia lie beside the nerve ring in the head. They each contain about (30) neuron cell bodies and send their neuronal processes into the ring at its posterior margin either laterally or ventrally via the amphid commissures and ventral ganglion, but form no local neuropil separate from the nerve ring. The lateral ganglia are in close contact with the lateral hypodermal cords.	lateral ganglion	lateral ganglia	WBbt:0005105
ganglion anterior to the nerve ring	anterior ganglion		WBbt:0005375
ganglion lies beside the nerve ring in the head, just anterior of the retrovesicular ganglion. It contains about 20 interneuron and motorneuron cell bodies that all send their neuronal processes into the ring. The cell bodies are divided into two groups by the intrusion of the excretory duct and canal. The cells are bounded by a basal lamina which physically separates them from the lateral ganglion even though they are adjacent to one another.	ventral ganglion	ventral ganglia	WBbt:0005298

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000453	ceh-32	W05E10.3	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010