WormMine: ExpressionPattern Expr877

• Pattern: GFP was detected in various nuclei starting from before gastrulation through adulthood. Newly hatched larvae expressed GFP in nuclei of the hypodermis (hyp3, hyp4, hyp6, and hyp7), intestine, pharyngeal muscle cells, and neurons (in the head and in the ventral, retrovesicular, preanal, and lumbar ganglia). In the second larval stage (L2), more hypodermal nuclei fluoresced in the body and the tail. In adults, GFP fluorescence was much fainter in the hypodermal cells and no longer delectable in some neurons in the head and tail. Other neuronal expression and the intestinal expression remained. GFP::EGL-44 was expressed in two cell types, the FLP and HSN cells, whose cell fate is altered in egl-44 mutants. The fluorescence in the FLP cells was maintained from the L1 larval stage through adulthood. In contrast to the FLP cells, which expressed the fusion postembryonically, the HSN neurons fluoresced only embryonically, at the 1.5-fold stage. Authors confirmed the identity of these embryonic HSN cells by noting the absence of this fluorescence in egl-1(n487) mutants, animals in which the HSN neurons die at this time. GFP::EGL-44 expression was not seen in the touch cells.
• Primary Identifier: Expr877
• Remark: A C-terminal GFP fusion (EGL-44::GFP) fluoresced in pharyngeal muscle cells and some intestinal nuclei. Because this reporter did not rescue the Egl phenotype, it may not reflect accurately the egl-44 expression pattern. A free C terminus may be important for function, because an N-terminal protein fusion (GFP::EGL-44) partially rescued the Egl phenotype, and authors believe the pattern of fluorescence is more likely to represent the true expression pattern of the gene.
• Subcellular Localization: nuclei.

15 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
Cylindrical hypodermal syncytium in head	hyp3	lineage name: ABplaapaaaa	WBbt:0004690
Cylindrical hypodermal syncytium in head	hyp4	lineage name: ABarpapapa	WBbt:0004687
Ganglion that lies at the anterior limit of the ventral nerve cord, near the ventral ganglion and nerve ring in the head posterior to the excretory pore. It is open and continuous with the region containing the motoneurons of the ventral cord. In the early L1 this ganglion holds 12 neuron cell bodies plus one neuroblast (Sulston and Horvitz, 1977; White et al., 1986). In the adult animal, the ganglion holds 20 neuron cell bodies.	retrovesicular ganglion		WBbt:0005656
ganglion in the head.	head ganglion		WBbt:0005135
ganglion anterior to the anus.	preanal ganglion		WBbt:0005448
The left and right lumbar ganglia lie behind the pre-anal ganglion in the tail, in lateral positions. Each contains 12 neuron cell bodies that send most of their neuronal processes into the ventral nerve cord via lumbar commissures and the pre-anal ganglion. There is no local neuropil in these ganglia in the hermaphrodite, instead they form most synapses in the pre-anal ganglion. Most lumbar neurons have sensory functions. In the adult male tail, these two ganglia gain many additional neurons and have some local neuropil. The lumbar neurons lie in close association with the lateral hypodermis, and most are posterior to the anus.	lumbar ganglion	lumbar lateral ganglia	WBbt:0005830
Cylindrical hypodermal syncytium in head	hyp6	lineage name: ABarpaapaa	WBbt:0004679
ganglion lies beside the nerve ring in the head, just anterior of the retrovesicular ganglion. It contains about 20 interneuron and motorneuron cell bodies that all send their neuronal processes into the ring. The cell bodies are divided into two groups by the intrusion of the excretory duct and canal. The cells are bounded by a basal lamina which physically separates them from the lateral ganglion even though they are adjacent to one another.	ventral ganglion	ventral ganglia	WBbt:0005298
Neuron, ciliated ending in head, no supporting cells, associated with ILso	FLPL	lineage name: ABplapaaapad	WBbt:0004798
Neuron, ciliated ending in head, no supporting cells, associated with ILso	FLPR	lineage name: ABprapaaapad	WBbt:0004797

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001208	egl-44	F28B12.2	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002