WormMine: ExpressionPattern Expr1614

• Pattern: At the beginning of embryonic morphogenesis, all ten pairs of lateral epidermal cells, the seam cells, express cdh-3::lacZ. cdh-3::GFP expression in these cells is observed at hatching and throughout subsequent postembryonic development. During the last larval stage (L4) the 15 pairs of seam cells generated during larval development fuse to form two continuous lateral syncytia, surrounded by hyp7. cdh-3::GFP expression correlates with seam cell identity during these postembryonic divisions; it is not observed in daughters that fuse with hyp7 or adopt other fates. In embryos undergoing morphogenesis, lacZ is expressed in a single large nucleus, whose size and position is consistent with that of the excretory cell. This identification is reinforced by the observation that in many newly hatched L1 larvae, low levels of GFP expression are visible in the excretory cell. This expression of cdh-3-reporter genes was observed only during late embryogenesis and in some newly hatched L1s (the latter may be due to perdurance of the GFP fusion protein), but not at later stages. Several other cells expressed the cdh-3 reporter constructs during embryonic morphogenesis; as in the seam cells this expression continued upon hatching. In the tail two cells, hyp10 and hyp11, show strong GFP expression that persists only during the first larval stage. Strong expression was observed in cells that form interfacial epithelia between the intestinal epithelium and the epidermis. Two cells that form part of the rectal epithelium, designated F and U, express cdh-3::GFP during embryonic morphogenesis and throughout larval development. In the anterior of embryos undergoing morphogenesis there were several cells expressing cdh-3::lacZ, and in larvae and adults, GFP expression is seen in nine cell bodies located just anterior to the first bulb of the pharynx. Processes extend anteriorly from these cell bodies and terminate at the level of the buccal capsule. Based upon the location of the cell bodies and the morphology of the processes, these cells were identified as the anterior and posterior arcade cells. Expression pattern of the pJP#38 construct in males indicated expression in the male tail and several male-specific neurons, however the expression pattern is complicated and the cell identity were not determined. cdh-3::GFP expressed in the developing hermaphrodite vulva. GFP first expressed in the anchor cell in L3 larvae. A little later expression were seen in those vulval cells that are closest to the anchor cell and are beginning to invaginate. As vulval morphogenesis continues all of the cells that invaginate to form the vulva are expressing GFP. During this period, the uterine epithelium closest to the invaginating vulval cells begins to express cdh-3::GFP and the anchor cell fuses with the multinucleate uterine seam cell (utse), which also begins to express cdh-3::GFP. Expression continues in these cells into the adult stage, though at somewhat reduced levels, which may perhaps be due to perdurance of the fusion protein, since older adults show much reduced fluorescence compared with younger ones. During the time that the vulva is forming, cdh-3::GFP expressed in the six VC neurons located in the ventral nerve cord and the two HSNs located just posterior and dorsal to the developing vulva. These cells begin to extend processes at about this time, and GFP expression continues in these cells and their processes throughout the remainder of larval development and into adulthood.
• Primary Identifier: Expr1614
• Remark: Reporter gene fusion type not specified.

49 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Neuron class of six motoneurons in the ventral cord, which innervate ventral body muscles and the vm2 muscles of the vulva.	VC neuron		WBbt:0005304
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC4	lineage name: P6.aap	WBbt:0004617
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC5	lineage name: P7.aap	WBbt:0004613
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
Tail ventral hypodermis	hyp10	lineage name: ABplppppppp	WBbt:0004378
Tail dorsal hypodermis	hyp11	lineage name: Cpappv	WBbt:0004377
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar.	vulF		WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr.	vulE		WBbt:0006767
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522
Seam hypodermal cell, postembryonic blast cell	H1L	lineage name: ABplaaappp	WBbt:0004766
Seam hypodermal cell, postembryonic blast cell	H1R	lineage name: ABarpapppp	WBbt:0004765
Seam hyopdermal cell, postemb. blast cell, L1 deirid socket	H2L	lineage name: ABarppaaap	WBbt:0004764
Seam hyopdermal cell, postemb. blast cell, L1 deirid socket	H2R	lineage name: ABarpppaap	WBbt:0004763
Seam hypodermal cell	H0L	lineage name: ABplaaappa	WBbt:0004778
Seam hypodermal cell	H0R	lineage name: ABarpapppa	WBbt:0004777
adult vulval toroid cell, C, formed by fusion of precursors of lineage names P5.ppal, P5.ppar, P7.papl and P7.papr.	vulC		WBbt:0006765
Interface between pharynx and hypodermis, form anterior part of the buccal cavity.	anterior arcade cell	arc ant	WBbt:0005794
adult vulval toroid cell, B1, formed by fusion of precursors of lineage names P5.papa and P7.ppap.	vulB1		WBbt:0006763
adult vulval toroid cell, B2.	vulB2		WBbt:0006764
adult vulval toroid cell, D, formed by fusion of precursors of lineage names P5.ppp and P7.paa.	vulD		WBbt:0006766
Interface between pharynx and hypodermis, form anterior part of the buccal cavity.	posterior arcade cell	arc post	WBbt:0005795
adult vulval toroid cell, A, formed by fusion of precursors of lineage names P5.paaa, P5.paap, P7.pppa and P7.pppp	vulA		WBbt:0006762
Tail seam hypodermal cell, postembryonic blast cell, functions as phasmid socket in L1	TL	lineage name: ABplappppp	WBbt:0004946
Tail seam hypodermal cell, postembryonic blast cell, functions as phasmid socket in L1	TR	lineage name: ABprappppp	WBbt:0004944
toroidal epithelial cell that make up the bulk of the uterus, four cells anterior and four cells posterior to the vulva.	uterine toroidal epithelial cell	ut cell	WBbt:0006784
Rectal cell, blast cell in male	F cell	lineage name: ABplppppapp	WBbt:0004799

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000395	cdh-3	ZK112.7	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002