WormMine: ExpressionPattern Expr10205

• Pattern: GFP- CED-10 localized strongly to the apical domain of intestinal epithelial cells (likely to the microvilli).
• Primary Identifier: Expr10205
• Subcellular Localization: GFP-CED-10 localized to intracellular puncta in the cytoplasm. The authors identified the intracellular puncta labeled by CED-10 as endosomes by performing a series of colocalization studies with a previously established set of intestine-specific compartment markers. CED-10 appeared specifically enriched on endosomes along the early and recycling pathway. Direct overlap of intestinal GFP-CED-10-labeled puncta and a subset of early endosomes marked by RFP-RAB-5 was observed. GFP-CED-10 showed the strongest colocalization with recycling endosome marker RFP-RAB-10, and displayed less overlap with later acting recycling endosome protein RFP-RME-1. Little overlap was observed between GFP-CED-10 and markers for late endosomes (GFP-RAB-7), the Golgi (MANS-GFP), or multi-vesicular bodies (GFP-HGRS-1/Hrs) indicating specificity in endosome-type associated with CED-10.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.	intestinal cell		WBbt:0005792

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000424	ced-10	C09G12.8	Caenorhabditis elegans

0 Life Stages