WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  LacZ staining is first detected in both Z1.pp and Z4.aa and is seen in Z1.ppp, Z4.aaa, Z1.ppa and Z4.aap. All four cells continue to express until the middle of the L2 stage during AC/VU decision and then expression is restricted to either Z1.ppp or Z4.aaa, with expression observed in presumptive VU (Z1.ppa and Z4.aap) but not in presumptive AC. This is consistent with GFP fluorescence observed. lin-12::lacZ staining disappears in the VUs; staining reappears in their daughters just after division. The level of lin-12::lacZ expression from early L2 until the Vulva Precursor Cells (VPCs) divide in the L3 is uniform in all 6 VPCs. lin-12::lacZ reporter is also expressed in all twelve of the granddaughters of the VUs. There appears to be a time during the early L3 stage when the VUs no longer express the lin-12::lacZ reporter gene. During L3, beta-gal activity is detected in two sheath cells in each gonad arm: sheath cells No. 1 (Z1.paaa, Z1.apa, Z4.pap, and Z4.appp). During early L4 stage, eight more sheath cells express the transgene: sheath cells No. 2 (Z1.paapaaa, Z1.appaaa, Z4.paappp, and Z4.appappp) and sheath cells No. 3 (Z1.paapaap, Z1.appaap, Z4.paappa, and Z4.appappa). Staining is almost always observed in sheath cells No. 1 in the L3 and L4 stages as well as in the young adult. Only a subset of animals consistently express the reporter gene in sheath cells No. 2. Staining in sheath cells No. 3 are never detected once the nuclei have migrated for out along the arm. Staining is observed in 12 sheath cells during the late L3/early L4 stages soon after they are born. As the cells move out the gonad arm, staining is only detected in one member of pair No. 1 and one member of pair No. 2 in each arm. Staining is detected during L4 in up to eight spermathecal cells [Z1.papaa(a/p) (d/v), Z4.apaaa(a/p)(d/v), Z1.pappp(a/p)(d/v) and Z4.apapp(a/p)(d/v)] in each arm. The progenitors of these cells [Z1.papaa(a/p), Z4.apaaa(a/p), Z1.pappp(a/p) and Z4.apapp(a/p)] also express the reporter gene. During the L2 and early L3 stages, lin-12::lacZ is expressed in all six VPCs. LacZ staining is detected in all 12 daughters of the VPCs (Pn.px stage), and is then restricted to P5.ppa, P5.ppp, P7.paa and P7.pap. High level of GFP expression is seen in the daughters of P5.p and P7.p but not in the daughters of p6.p. Variable level of GFP is detected in the daughters of P3,p, P4.p and P8.p. In the VPC granddaughters GFP is detected in P5.ppa, P5.ppp, P7.paa and P7.pap. Weak staining is detected in two of the granddaughters of P5.p and P7.p, the L cells, but is undetected in their progeny. Expression is always detected in the other two granddaughters of P5.p and P7.p, the N and T cells. There is no staining in the T descendants of P6.p cells. The N cells do not divide and staining is detected in both N cells throughout vulval morphogenesis in the L4 stage and in young adults. Staining can be detected in the T daughters in the L4 stage and in young adults. The parents of the SM/bm precursor cells; M.vlp and M.vrp and their dorsal equivalents, M.dlp and M.drp all express lin-12 reporter gene. After division of the parent cells, the SM/bm precursor cells and their dorsal equivalents also express the reporter gene as do the sisters of these cells. Expression is detected in both the SM and bm cells on the left and right sides of the animal. Staining persists in these cells on the ventral side even after it is no longer detectable in the cells of the dorsal side. [M.vrpa, M.vlpa, M.vrpp, M.vlpp, M.drpa, M.dla, M.drpp and M.dlpp]. Expression is detected in a discrete subset of cells during embryogenesis. Staining was only observed in pairs or groups of cells from the 28-cell stage to about the 400-cell stage. Two of the cells that express the gene in the >300-cell embryo may be the intestinal valve cells. Expression is seen in a discrete subset of cells in the ventral nerve cord of L1 larvae. There are three small nuclei that stain in the head region that may be G2, W, the excretory duct cell, G1 or the neuroblast that is the equipotent equivalent of the excretory cell. Staining was observed in the excretory cell. Primary Identifier  Expr631
Remark  This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).
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89 Anatomy Terms

Definition Name Synonym Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772
an accordion-like tube that contains sperm and is the site of oocyte fertilization. spermatheca   WBbt:0005319
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring. ventral nerve cord ventral cord WBbt:0005829
The extracellular sinus formed by the excretory duct and pore cells. Excretory duct   WBbt:0005777
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite. P5.p hermaphrodite   WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite. P4.p hermaphrodite   WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite. P3.p hermaphrodite   WBbt:0008112
  P8.p hermaphrodite   WBbt:0008133
  P7.p hermaphrodite   WBbt:0008129
  P6.p hermaphrodite   WBbt:0008125
Posterior daughter cell of P8.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P8.pp lineage name: P8.pp WBbt:0004145
Posterior daughter cell of P3.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P3.pp lineage name: P3.pp WBbt:0004153
Anterior daughter cell of P4.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P4.pa lineage name: P4.pa WBbt:0004151
Posterior daughter cell of P4.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P4.pp lineage name: P4.pp WBbt:0004149
Anterior daughter cell of P8.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P8.pa lineage name: P8.pa WBbt:0004147
Posterior daughter cell of P5.pp, fuses into hermaphrodite vulD syncytium. P5.ppp lineage name: P5.ppp WBbt:0004477
Anterior daughter cell of P7.pa, fuses into hermaphrodite vulD syncytium. P7.paa lineage name: P7.paa WBbt:0004448
Anterior daughter cell of P3.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite. P3.pa lineage name: P3.pa WBbt:0004155
cell, anterior daughter of P6.p P6.pa   WBbt:0006987
cell, posterior descendent of P7.p P7.pp   WBbt:0006984
cell, anterior descendent of P7.p P7.pa   WBbt:0006983
cell, posteior daughter of P6.p P6.pp   WBbt:0006988
post-embryonic cell of pedigree P5.pp P5.pp   WBbt:0007255
post-embryonic cell of pedigree P5.pa P5.pa   WBbt:0007252
post-embryonic cell of pedigree P7.pap P7.pap   WBbt:0007264
post-embryonic cell of pedigree P5.ppa P5.ppa   WBbt:0007256
adult vulval toroid cell, C, formed by fusion of precursors of lineage names P5.ppal, P5.ppar, P7.papl and P7.papr. vulC   WBbt:0006765
adult vulval toroid cell, D, formed by fusion of precursors of lineage names P5.ppp and P7.paa. vulD   WBbt:0006766
Post-embryonic cell of pedigree M.vlpp. M.vlpp mu_bod WBbt:0007190
post-embryonic cell of pedigree M.vlpa M.vlpa   WBbt:0007183

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003001 lin-12 R107.8 Caenorhabditis elegans

6 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. embryo Ce WBls:0000003