WormMine: ExpressionPattern Expr2543

• Pattern: In adult worms, UNC-60A was highly expressed in the intestine, germ cells in the distal gonad, oocytes and spermatheca but not in body wall muscle. UNC-60B was expressed in body wall muscle, vulva and spermatheca. Both ADF/cofilin isoforms were not detected in the smooth-muscle-like myoepithelial sheath cells. It was previously demonstrated that UNC-60B is not expressed in early embryos and become detectable from the comma to 1.5-fold stages (approximately 290 minutes after first cell division) only in the body wall muscle cells. UNC-60A was maternally expressed and present in most of embryonic cells throughout embryogenesis. In the later stages, staining of UNC-60A in the nerve ring became intense.
• Primary Identifier: Expr2543
• Subcellular Localization: At the one-cell stage, UNC-60A was diffuse in the cytoplasm and localized to no particular structure. Actin was localized throughout the cortex as well as in the diffuse cytoplasm and assembled at the early cleavage site in the telophase of the first mitosis, whereas UNC-60A did not significantly accumulate in these structures. During progression of cytokinesis and after the two-cell stage, association of UNC-60A with the cortical regions, especially at the cell-cell boundaries became evident. In addition, UNC-60A was colocalized with actin to dot-like structures at the anterior cortex of each blastomere. These are believed to be remnants of cell division. UNC-60B is diffusely localized in the cytoplasm of the embryonic body wall muscle in the later stages. In adult worms, localization of the nuclei of the gonad by DAPI indicated that both UNC-60A and UNC-60B were mostly localized to the cytoplasm. Also, they were undetectable in sperm, which does not have an actomyosin system.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
female genital.	vulva		WBbt:0006748
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006794	unc-60	C38C3.5	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003