WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  The transgene containing C06A5.8 3' UTR expressed GFP::H2B in oocytes and the meiotic region, but was either absent or very low in the mitotic germ cells. Primary Identifier  Expr9711
Reporter Gene  We employed a transgene-based assay that tests the post-transcriptional control activity of 3' UTRs in vivo. In this assay, the GFP::H2B reporter fusion is placed upstream of the selected 3' UTR such that the entire fusion-GFP::H2B-3' UTR-is produced as a single transcript. Expression in the germline is achieved by using pie-1 promoter, which has been shown earlier to drive transgene expression in the germline.

2 Anatomy Terms

Definition Name Synonym Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes. germ line germline WBbt:0005784
a female haploid germ cell. oocyte ovum WBbt:0006797

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00015505 C06A5.8 C06A5.8 Caenorhabditis elegans

0 Life Stages