WormMine: ExpressionPattern Expr12840

• Pattern: C. elegans nematodes that were genetically engineered to express GFP under the control of the zmp-2 promoter showed expression of zmp-2 earliest during embryogenesis in laid eggs. During the organogenesis/morphogenesis stage zmp-2 expression starts and multiple cells showed strong fluorescence in a complex manner in the early comma stage, the 1.5-fold stage, and the 3-fold stage. During larval development zmp-2 expression was concentrated in specific muscle and somatic gonad cells. In L4 larvae we observed strong signals in the developing spermatheca and weak signals in vulval muscles. In hermaphroditic adults the strongest expression was detectable in the two spermathecae and spermathecal-uterine valves. Anal cells as well as two cells in the head showed weaker expression. The head cells may represent motor neurons RMEV and RMED according to their position. Additionally, diffuse hypodermal GFP expression was observed in larvae shortly before molting events as well as in resting dauer larvae that start feeding and leaving their diapause. Within 1 h of accessing food, the animals usually exit the dauer stage and after further 8-9 h they molt to the L4 stage. Here, we determined strong hypodermal zmp-2 promoter regulated GFP expression of transgenic nematodes after 6.5-7 h after access to food shortly before molting process, providing further evidence for a role of ZMP-2 in ecdysis.
• Primary Identifier: Expr12840

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
Ring motor neuron	RMED	lineage name: ABalapppaap	WBbt:0005027
Ring motor neuron	RMEV	lineage name: ABplpappaaa	WBbt:0005021
The components of the gonad that are separate from the germline proper. In hermaphrodite, these include five tissues which are all derived from the somatic primordium : the distal tip cells, the gonadal sheath, the spermatheca, the spermatheca-uterine valve (sp-ut) and the uterus.	somatic gonad	somatic germline	WBbt:0005785
A single syncytium cell of four nuclei makes a valve, connecting spermatheca and uterus of hermaphrodite gonad.	spermathecal-uterine valve cell	sp-ut cell	WBbt:0008217
body region surrounding the anus.	anal region		WBbt:0006919

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00019212	zmp-2	H19M22.3	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A third stage larva specialized for dispersal and long term survival.		dauer larva Ce	WBls:0000032
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019