WormMine: ExpressionPattern Expr9861

• Pattern: GFP expressed from the lin-42a promoter was robustly expressed in the pharyngeal myoepithelium midway through the L1 stage and continuously thereafter. The lin-42a reporter was also expressed in hyp7 and the lateral seam cells of late L1-stage larvae undergoing the first molt. Expression of the lin-42a reporter in the hypodermis was detected later in the L1 stage than expression of the lin-42b reporter. Fluorescence from the lin-42a reporter also persisted through ecdysis, whereas fluorescence from the lin-42b reporter dissipated during lethargus. To fully define the cyclical expression pattern of the lin-42ap::gfp-pest fusion gene, we measured the relative intensity of the associated fluorescence in the hypodermis throughout larval development. The intensity of fluorescence repeatedly increased 2- to 10-fold during the molts and dropped to background levels early in the next life stage.
• Primary Identifier: Expr9861
• Reporter Gene: [lin-42ap::gfp-pest] transcriptional fusion. The start codon of lin-42a and 3,043 bps of upstream sequence were fused with gfp-pest. --precise ends.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00018572	lin-42	F47F6.1	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage when an animal shifts from L4 larva to adult. It includes the synthesis of new cuticle, cease of phrayngeal pumping during a lethargus stage, and the shed off of old cuticle.		L4-adult molt Ce	WBls:0000040
	The stage when an animal shifts from L2 larva to L3 larva. It includes the synthesis of new cuticle, cease of phrayngeal pumping during a lethargus stage, and the shed off of old cuticle.		L2-L3 molt Ce	WBls:0000029
	The stage when an animal shifts from L1 larva to L2 larva. It includes the synthesis of new cuticle, cease of phrayngeal pumping during a lethargus stage, and the shed off of old cuticle.		L1-L2 molt Ce	WBls:0000026
	The stage when an animal shifts from L3 larva to L4 larva. It includes the synthesis of new cuticle, cease of phrayngeal pumping during a lethargus stage, and the shed off of old cuticle.		L3-L4 molt Ce	WBls:0000037