WormMine: ExpressionPattern Expr2735

• Pattern: Expressed in early embryos. The staining in the embryos was specific because it was largely reduced in syn-4(RNAi) embryos compared with wild-type embryos. In L1-stage larvae, prominent staining was detected in two rows of cells, which appeared to be the cuticle-secreting seam cells. In the adult hermaphrodite, Syn-4 was largely confined to the gonad.
• Primary Identifier: Expr2735
• Subcellular Localization: An identical pattern of gonadal staining was observed in an adult expressing a GFP::Syn-4 fusion construct. Syn-4 was present in the outer membrane of early embryos. During cytokinesis, Syn-4 staining was observed in furrows that had just begun to ingress and as the membrane invaginated, the extent of Syn-4 staining expanded. Syn-4 remained associated with the membranes that separate blastomeres. The punctate staining pattern observed in the cytoplasm of early embryos probably reflects vesicular structures, which were often enriched around the forming nuclei. In the adult hermaphrodite, Syn-4 was largely confined to the gonad, where it localized to the incomplete membranous structures that separate the germ cell nuclei.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
organ producing either sperm or ova.	gonad		WBbt:0005175

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006374	syx-4	T01B11.3	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057