WormMine: ExpressionPattern Expr12249

• Pattern: mir-237, was not expressed in the seam cells until eL3, which mirrored the up-regulation of this gene at this stage by northern blot. mir-237, was detected two stages earlier at L1 in the somatic gonad progenitor cells, Z1 and Z4. This result differs from our northern blot data, which showed that miR-237 RNA was first detected at L2 (Expr3739). We believe that the mir-237::gfp expression at the L1 stage is accurate, and since mir-237 is only expressed in the Z1 and Z4 cells at this stage, miR-237 RNA is below the levels of detection by northern blot analysis. Once the Z1 and Z4 somatic gonad derivatives expand at L2, miR-237 RNA can be seen by northern blot. By the L3 stage, and persisting into the adult, mir-237 was expressed in the developing gonad, including the anchor cell, a subset of unidentified uterine cells, and the distal tip cells. The up-regulation of mir-237 expression in animals carrying the mir-237::gfp construct at the L3 stage in a variety of tissues, such as the gonad and the hypodermis, mirrors the increased miR-237 RNA levels observed at L3 by northern blot analysis. In addition, mir- 237 and mir- 84, were both expressed in the anchor cell and in the VPCs except P6.p at the mL3 stage. It is interesting to note that expression of mir-237, mir-84, and mir-48 was absent in the P6.p cell during the mid- to late L3 stage, and expression of mir-237 and mir-84 but not mir-48 was present in the anchor cell.
• Primary Identifier: Expr12249

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
somatic cell that situates at the tip of a gonad arm.	distal tip cell	DTC	WBbt:0006865
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809
Somatic gonad precursor cell	Z1	lineage name: MSpppaap	WBbt:0004577
Somatic gonad precursor cell	Z4	lineage name: MSappaap	WBbt:0004574
immature gonadal structure of hermaphrodite.	developing hermaphrodite gonad		WBbt:0006903

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003330	mir-237	F22F1.4	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035