WormMine: ExpressionPattern Expr3089

• Pattern: GFP expression was seen in hypodermal seam cells. This expression began in the mid-L1 stage and was present in all larval stages and the adult. GFP expression was seen in sheath cells of the somatic gonad. Expression was also seen in a subset of ventral cord neurons (VCNs) and in the anchor cell of the gonad that was not previously observed with antibody staining or a BAR-1 translational GFP reporter. Expression is seen in 7 to 15 neurons between the posterior bulb of the pharynx and the anus in L1 animals 1 h after hatching, indicating that some embryonically derived neurons in the ventral cord are expressing bar-1. During the rest of the L1 and L2 stages, the number of GFP-expressing neurons increases to 20 40 per animal. By the L4 stage, the number of ventral cord neurons showing GFP expression anterior to the vulva is 0 9, and posterior to the vulva is 11 24. In regard to vulval development, GFP expression from the transcriptional fusion was first seen in Pn.p cells after their birth in the L1 stage. This expression persisted in the L2 and L3 stages and continued throughout the divisions of the VPCs until the early L4 stage. In the adult, strong GFP expression is confined to the vulval muscles, but faint expression is still seen in adult vulval cells.
• Primary Identifier: Expr3089

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
female genital.	vulva		WBbt:0006748
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000238	bar-1	C54D1.6	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035