WormMine: ExpressionPattern Expr11268

• Pattern: Four constructs were made where GFP was fused to either initiation site or stop codon in order to determine if the splice variants are differentially expressed. Expression of the transformation vectors was driven under 1,951 bp of the KCNL-2 endogenous promoter. Confocal fluorescent imaging of transgenic lines expressing these constructs in the N2 background showed a neuronal expression pattern. expression of KCNL-2 when tagged at the latter stop codon (pkcnl-2kcnl-2(taa2)::gfp) was detected in head neurons, the nerve ring (NR), motor neurons of the ventral nerve cord (VNC), the dorsal cord (DC) and tail ganglia. The pkcnl-2kcnl-2(taa2)::gfp construct was expressed in many neuronal processes innervating the vulva while pkcnl-2gfp::(atg2)kcnl-2 shows expression in the VC4 and VC5 neurons of the egg-laying apparatus in addition to other neuronal processes innervating the vulva. The authors made two additional constructs to drive GFP expression under the KCNL-2 promoter to aid in determining the complete expression profile of KCNL-2 that may be masked in transgenic lines that express GFP-tagged KCNL-2.The first construct, pkcnl-2(atg1) gfp, encompassed the coding sequence of GFP which was expressed downstream of the promoter of KCNL-2 (1,951 bp upstream of atg1). The second construct, pkcnl-2(atg2) gfp, expressed GFP at the second initiation site where the coding sequences for kcnl-2-b and -c were deleted. pkcnl-2(atg1) gfp showed a neuronal expression profile with additional GFP-expression in the vulval muscles. pkcnl-2(atg2) gfp showed a strict neuronal expression profile that complemented the expression profile of the pkcnl-2kcnl-2::gfp constructs. pkcnl-2(atg2) gfp readily labels the VC4 and VC5 neurons and displays a highly innervated vulva, a feature that is lacking in the expression profile of pkcnl-2(atg1) gfp. Ultimately, since both pkcnl-2(atg1) gfp and pkcnl-2(atg2) gfp are expressed in different neurons, this suggests that the various isoforms of KCNL-2 may have differing neuronal expression profiles.
• Primary Identifier: Expr11268

11 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
a bundle of nerve processes that runs along the dorsal mid-line of the animal.	dorsal nerve cord	dorsal cord	WBbt:0006750
Seam hypodermal cell, postembryonic blast cell	V4L	lineage name: ABarppappa	WBbt:0004894
Seam hypodermal cell, postembryonic blast cell	V4R	lineage name: ABarpppppa	WBbt:0004892
Seam hypodermal cell, postembryonic blast cell	V5L	lineage name: ABplapapaap	WBbt:0004890
Seam hypodermal cell, postembryonic blast cell	V5R	lineage name: ABprapapaap	WBbt:0004876
ganglion in the tail region (posterior to rectum).	tail ganglion		WBbt:0006977

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00008570	kcnl-2	F08A10.1	Caenorhabditis elegans

0 Life Stages