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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr660
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9h after fertilization: strong staining in intestinal and hypodermal nuclei; Weak neuronal staining. Early L1: staining in nuclei of most postembryonic blast cells. Strong staining in nuclei of hypodermic blast cells H1, H2, V1-V6, T and all intestinal (E) cells. Weak staining nuclei of neuroblasts Q1 and Q2, mesoblast M cells and P cells. 9h after fertilization: strong staining in intestinal and hypodermal nuclei; Weak neuronal staining. Early L1: staining in nuclei of most postembryonic blast cells. Strong staining in nuclei of hypodermic blast cells H1, H2, V1-V6, T and all intestinal (E) cells. weak staining nuclei of neuroblasts Q1 and Q2, mesoblast M cells and P cells. Adult: staining observed in the mature oocyte nuclei of hermaphrodites, at meiotic prophase I when the chromosomes are condensed. (Possible artifact, detected in lin-14 loss-of-function mutant strains n536n540, n355n726). In embryo, first observed in embryo at 7h after fertilization (half way through embryogenesis). Strong staining in intestinal and hypodermal nuclei. L3: Pn.p stains weakly before division (staining fades by L4). Occasional weak staining of hypodermal, intestinal and neuronal nuclei and cytoplasm at L2 and L3. Late L1: staining of all nuclei except for neuronal nuclei is weaker. More neuron of the nerve ring and posterior ganglion stain than in earlier stages. Intestinal and hypodermal cell lineages stain strongest at mid to late L1 (Fade entirely by L2) similarly with many of the neuronal cells. Mid L1: staining in nuclei of hypodermic blast cells H1, H2, V1-V6 and T. The nuclei of intestinal (E) cells also stain. Weak staining in nuclei of P cells (staining fades before migration into ventral cord). Strong staining in nuclei of embryo-derived nuclei in hypodermal syncytial cell hyp7, ABarpppapa, ABplaapppp, Cpaaaa, Cpaapa, Cpaapp, Cpapaa, terminally differentiated nuclei from embryonic body muscle also stain for lin-14. Staining observed in nuclei of neuronal cells BDU, ALM, and CAN. All embryonic generated ventral cord neurons and some neurons of the nerve ring and posterior ganglion stain for lin-14. |
lin-14 is localized to the nuclei. |
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Lineage expression: H, V, T descandents. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr661
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lin-14 protein is first observed in embryos at ~7 hours after fertilization where most intense staining is seen in intestinal and hypodermal nuclei. ~9 h after fertilization, additional weak staining is observed. lin-14 protein is expressed at high level in the nuclei of most of the post-embryonic blast cells. Intense nuclear staining was observed in the hypodermal blast cells H1, H2, V1-V6 and T and in all of the intestinal (E) cells and weaker staining was observed in both neuroblasts Q1 and Q2, in the mesoblast M cell and in P cells (P1/2, P3/4 and P5/6). During L1, staining is seen in the progeny of the hypodermal blast cells H1, H2 V1-V6 and T and in all of the intestinal (E) cells. Staining in P-cell nuclei fades before migration into the ventral nerve cord but reappears later in some of their progeny cells. The embryo-derived nuclei in the hypodermal syncytial cell hyp7, ABarpppapa, ABplaapppp, Cpaaaa, Cpaapa, Cpaapp, Cpapaa, all stain for the lin-14 protein during the L1 stage. Terminally differentiated nuclei from embryonic body muscle also accumulate the lin-14 protein. Nuclei of many but not all neuronal cells stain with the antibody (e.g. BDU, ALM, CAN but not HSN). All of the embryonically generated ventral cord neurons and some but not all of the neurons of the nerve ring and the posterior ganglion accumulate the lin-14 protein in their nuclei during the L1 stage. Late L1 stage, staining is seen in all nuclei except in the neuronal nuclei staining is much weaker. In addition, more neurons of the nerve ring and posterior ganglion stain than at the earlier stages. Thus, in the hypodermal and intestinal cell lineages, lin-14 protein level peaks during early L1 and fade entirely by L2. In the many neuronal cells, lin-14 protein peak during mid to late L1 and fade by L2. Pn.p accumulates lin-14 protein at the L3 stage, although, very weak staining is seen before the Pn.p cells divide. This staining fades by early L4, In occasional L2 and L3 stage animals, weak staining is observed in nuclei and cytoplasm of hypodermal, neuronal and intestinal cells. Patches of staining in hypodermal or intestinal nuclei is only rarely observed in very old adults. In most adults, staining reappears only in the mature oocyte nuclei of hermaphrodites at meiotic prophase I when the chromosomes are condensed. The oocyte nuclear staining disappears after fertilization. Quantitation of immunoblots show that the level of lin-14 protein relative to a pharyngeal myosin control decreases >= 25-fold from L1 to L2. |
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Other strain-- UL123 |
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Expr103
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This strain exhibits strong expression in the embryo. Expression is first seen in the 50-80 cell embryo and extends through to adulthood. It appears that most of the AB cells in the embryo stain, and what appears to be the cells of the C lineage. Some embryos exhibit staining in the two rows of nuclei that are the E lineage. All embryonic staining is very intense, and it spreads to the cytoplasm giving blue embryos, therefore obscuring the DAPI staining, making it difficult to count the number of cells in the embryos as each component begins expressing. This intense staining fades as the embryo ages, sometimes leaving blue comma stage embryos with no distinct nuclei staining. Hypodermal expression is seen in the 3 fold stage of embryogenesis and in young larvae which most probably are C-derived hyp-7 nuclei. Expression weakens as the worm gets older and is much less frequently expressed in adults. Some adults do show staining in the anterior hypodermal nuclei (hyp-3, hyp-4) and in the anterior hypodermal seam cells, also some nuclei stain in the tail. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr706
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NHR-2 is detected in the nuclei of embryos as early as 2-cell stage. The protein is present in every nucleus until the 16-20 cell stage then no longer detected in germline precursor P4 and its sister D but at this point expression in other cells increase. No staining during or just after mitosis. 28-cell stage: Staining in E and MS descendants, variable expression generally weak particularly in E cells. Staining in ABplp and ABpr descendants also variable but can be quite strong. The other 10 AB cells and 4 C cells exhibit reproducible strong expression. 51-cell stage: No expression in descendants of E. Staining in C cells, many AB cells and some MS cells (particularly those in anterior and dorsal positions). As embryogenesis progresses NHR-2 expression is restricted to anterior and dorsal regions of embryo. 250 cell stage: Nuclei staining include (but not limited to) Cp descendants contributing to hyp7 synctium, many but not all AB descendants. NHR-2 last detected in one or a few nuclei in vicinity of excretory cell before expression ceases at early comma stage. |
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Expr3279
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In the embryo, the downstream promoter (ten-1b) is most active in the descendants of the ABp cell and in the hypodermis. The dorsal hypodermal cells and the ventral leader cells were most prominently labeled. During postembryonic development, GFP fluorescence was visible in specialized epithelial cells including the arcade cells of the anterior end and the excretory duct. Ten-1b is also active in a subset of neurons including CAN and HSN neurons as well as neurons of the lumbar and retro-vesicular ganglion and some nerve ring interneurons. In males, GFP fluorescence is also visible in R8 and R9 ray neurons. |
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Expr1633
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First, pKK52 expression begins at the 28-cell stage in all four granddaughters and 16 great-great granddaughters of the MS and AB founder cells, respectively; this expression continues in many, possibly all, of their descendants until around the time of hatching. Second, expression becomes more pronounced in seam cells about 1 hour after their birth. This seam expression remains strong throughout embryonic and larval development, but becomes slightly reduced in adults. Third, robust expression is also seen in several cells in the head region, at least some of which are cells in the nervous system (neurons and/or support cells), beginning at approximately the comma stage and continuing through adulthood. For simplicity, this component of the expression pattern was referred as nervous system expression, although the precise identity of these cells were not determined. |
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See Expr1633 for pKK52 expression pattern. |
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Expr1634
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pKK41 is expressed in the same groups of cells as the elt-5 translational reporter (pKK52), but the relative expression levels are different. Whereas the elt-5 reporter is strongly expressed in both seam cells and the nervous system during the comma through pretzel stages, the elt-6 reporter is strongly expressed only in the nervous system. Only weak expression of the elt-6 reporter is apparent in seam cells and in the AB and MS descendants during embryogenesis, but the seam expression becomes stronger during larval development. Strong expression of the elt-6 reporter in the nervous system continues throughout larval development. |
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Picture: Fig 2A. |
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Expr9010
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The INA-1::GFP protein was localized to the cell surface of the ABplaapppp cell. When the engulfment process was initiated, INA-1::GFP accumulated at the contact site with C3, even before C3 displayed a characteristic apoptotic morphology under DIC optics. |
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Expr10312
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10267
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10348
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10223
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10292
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10307
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10250
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10253
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10266
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10275
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10374
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10378
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10509
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Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10528
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Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10291
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10249
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10214
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Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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Expr10475
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Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/ |
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