WormMine: ExpressionPattern Expr3876

• Pattern: Around the mid L3 stage, GFP expression was also seen in two sex myoblasts located in the mid body region. Expression was highly penetrant and persisted in the sex myoblast descendants as they divided. GFP expression in these cells faded in the L4 stage and was not seen in differentiated vulval or uterine muscles. After the P cells divided in the L1, GFP expression can be observed in a subset of ventral cord neurons (VCNs). Expression in some neurons along the ventral cord persisted during the L2 stage, became more penetrant in the L3 stage, and remained throughout development even in the adult. Expression was also seen in a neuron in the head, which was reported previously. Authors did not examine GFP expression in the Q lineage, or in males. GFP expression was detected in P3/4 only when deIs4 was moved into a smg-1 mutant, in which nonsense-mediated RNA decay is compromised, suggesting that either there is less transcription of lin-39 in P3 and P4 (and their descendants), or more nonsense-mediated decay of the transcript from deIs4 in those cells. Once the P cells divided, GFP expression was observed in 50% of P3.p cells, 70% of P4.p cells, and 100% of P5.p to P8.p cells in smg-1; deIs4 (n>30) with similar levels of expression in P5.p to P8.p. Other lin-39 reporters also show this lower level of expression in P3.p and P4.p. This pattern of expression persisted throughout the L2 stage until the time of vulval induction in the L3 stage. At this time (29 h, post-hatching), GFP expression was stronger in P6.p than the other VPCs, showing that lin-39 upregulation occurred with these reporters. When the induced VPCs (P5.p to P7.p) start dividing, GFP expression remained in their daughters, with higher levels of GFP in P6.p descendants and lower levels in P5.p and P7.p descendants, even after the second and third divisions. In addition, increased GFP expression was seen during the late L3 stage in P5.paax and P7.pppx, the cells that fuse to form the VulA vulval cell, and this pattern remained until the late L4 stage. During vulval morphogenesis in the L4 stage, GFP was maintained in the 22 cells that form the vulval invagination only in the smg-1 background. GFP expression were detected in the P cells, VPCs, ventral cord neurons (VCNs), sex myoblasts and their descendants, and a head neuron shown to express lin-39. GFP expression were first detected early in the embryo in cells likely to be P5 to P8. This expression continued during embryogenesis and after hatching until the division of the P cells in the L1 stage.
• Primary Identifier: Expr3876

21 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite.	P5.p hermaphrodite		WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite.	P4.p hermaphrodite		WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite.	P3.p hermaphrodite		WBbt:0008112
	P8.p hermaphrodite		WBbt:0008133
	P7.p hermaphrodite		WBbt:0008129
	P6.p hermaphrodite		WBbt:0008125
The tenth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P10		WBbt:0006779
The ninth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P9		WBbt:0006778
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1	P11	lineage name: ABplapappa	WBbt:0004410
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1	P12	lineage name: ABprapappa	WBbt:0004409
The sixth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P6		WBbt:0006775
The fifth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P5		WBbt:0006774
The fourth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P4		WBbt:0006773
The third (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P3		WBbt:0006772
The eighth (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P8		WBbt:0006777
The seventh (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P7		WBbt:0006776
The second (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P2		WBbt:0006771
The first (count from anterior-most) of twelve postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1.	P1	P1 post-embryonic blast cell	WBbt:0006770
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809
Hermaphrodite-specific blast cell which divides to product muscle cell.	hermaphrodite sex myoblast		WBbt:0008605

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003024	lin-39	C07H6.7	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003