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Reporter gene fusion type not specified. |
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Expr3364
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Expressed in male seminal vesicle and vas deferens. |
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Expr12420
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K09C8.2::GFP labels the male seminal vesicle and vas deferens, but it is not expressed in wild-type hermaphrodite gonads. |
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Expr13980
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In males, DDO-3::mCherry was localized in the excretory pore cell, head tip cells, hypodermal cells, seminal vesicle cells, tail cells, and spicule cells. By contrast, DDO-3::mCherry was also localized in the tail fan from the adult stage, suggesting that DDO-3 is secreted in a SP-dependent manner from the cells where it is produced and transferred to the tail fan. Interestingly, DDO-3::mCherry was localized in vesicle-like structures in the seminal vesicle, where spermatids are stored until ejaculation. Because the seminal vesicle is composed of 20 secretory cells, we presumed that DDO-3 was secreted from the seminal vesicle into the seminal fluid and transferred to the hermaphrodite during mating. To test this possibility, we examined the uteri of hermaphrodites just after mating with transgenic males carrying the ddo-3::mCherry reporter gene. DDO-3::mCherry was dispersed throughout the uterus, suggesting that DDO-3 is a seminal fluid protein. Taken together, these results are consistent with the idea that DDO-3 is secreted from the seminal vesicle into the seminal fluid in a SP-dependent manner, and then transferred to the hermaphrodite uterus through mating. This fusion protein was also present in coelomocytes of males. During larval stages L1 and L2, localization of DDO-3::mCherry outside coelomocytes was limited to the excretory pore cell, implying that DDO-3 expressed in excretory pore cells is secreted into the body fluid. Indeed, at least in the adult stage, DDO-3::mCherry in excretory pore cells was localized to vesicle-like structures. |
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Expr13036
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ins-31 is expressed in the seminal vesicle in vesicular structures. INS-31 protein was also found in coelomocytes in males. |
ins-31 is expressed in the seminal vesicle in vesicular structures. |
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Expr13978
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In males, Pddo-3::mCherry was expressed in the excretory pore cell, head tip cells, and hypodermal cells, in a pattern similar to that observed in hermaphrodites. Moreover, this reporter protein was expressed in seminal vesicle cells from L4 and in tail cells and spicule cells from the adult stage. |
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Expr9988
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GFP expression was not observed in the intestine of transgenic worms expressing the Pddo-3::GFP construct. In these worms, GFP expression appeared in probable head mesodermal cells and unidentified cells in the head during the L3 to adult stages, in vulval muscles during the L4 to adult stages, and in the hypodermis and gonad sheath cells only during the adult stage. In the gonad sheath cells, the GFP signal in the U-shaped region was much brighter than in the other regions. In male worms expressing the Pddo-3::GFP construct, GFP expression was observed in probable head mesodermal cells and unidentified cells in the head during stages L3 to adult, and in hypodermis, seminal vesicle and tail cells only during the adult stage. Thus, a sex-specific difference was observed in DDO-3 expression and localization patterns. |
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Expr9862
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The primary site of try-5 expression was in the male somatic gonad, in particular within tissues involved in storing sperm and tissues through which sperm pass during transfer to a hermaphrodite. Starting at the L4 larval stage, when sperm production initiates, we observed Ptry-5::GFP::H2B expression in several regions of the male gonad: the seminal vesicle (up to seven of the twenty-three total cells in this tissue), the valve region (four cells), and the twelve cuboidal cells of the vas deferens. This overall pattern persisted into adulthood until at least 72 hr post L4; highest expression levels were present consistently in the valve region. We observed no expression in the hermaphrodite gonad, so gonadal expression of try-5 is sexually dimorphic. However, we also observed low levels of expression in a few cells within the head and tail of both males and hermaphrodites. |
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Expr3702
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Expression in male-specific structures is directed by all three promoters. Promoter pA directs expression in the spicule protractor muscles of the proctodeum and in a single male-specific neuron that is likely to be CP8 or CP9. Promoter pB directs expression in the spicule retractor muscles, gubernaculum retractor muscles, posterior oblique muscles, diagonal muscles, and the vas deferens. Promoter pC also directs expression in the vas deferens, as well as the seminal vesicle and the valve that separates the seminal vesicle and vas deferens. Thus itr-1 is expressed widely in the sex-associated muscles and somatic gonad of males. |
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Expr9863
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In worms carrying the Ptry-5::TRY-5::GFP reporter, the TRY-5::GFP fusion protein exhibited a localization pattern consistent with secretion from the vas deferens. Within the valve and cuboidal cells, TRY-5::GFP was localized to globular foci. In L4 larvae, most globules aligned with the apical domain that lines the developing sperm channel. In mature adults, very large globules were present that tended to cluster apically, and additional small globules were present throughout the cytoplasm. We sometimes observed TRY-5::GFP within the lumen of the seminal vesicle, likely as a result of release from the adjacent, highly-expressing valve cells. |
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Expr14565
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We generated transcriptional reporters for two of the uncharacterized genes (F17E9.3 and Y110A2AL.7) and confirmed that both are specifically expressed in the seminal vesicle. |
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Reporter gene fusion type not specified. |
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Expr3365
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Expressed in male seminal vesicle valve. |
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Expr14566
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We generated transcriptional reporters for two of the uncharacterized genes (F17E9.3 and Y110A2AL.7) and confirmed that both are specifically expressed in the seminal vesicle. |
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