1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00002285 | let-7 | C05G5.6 | Caenorhabditis elegans |
| Pattern | Fluorescence was observed at the end of the L1 stage in transgenic worms that express GFP fused to the pri-let-7B start site. Detection of GFP mRNA, driven by both let-7 promoter A and B sequences in the transgenic worms, mirrored that of endogenous let-7 primary transcripts, indicating that expression of let-7 is repressed largely at the transcriptional level from embryogenesis until the late L1 stage. Consistent with the reporter analysis, pri-let-7 was first observed during the late L1 stage. We detected all three pri-let-7 isoforms, and coordinate expression of these isoforms oscillated throughout development. The low levels of pri-let-7 at most mid-larval time points and the slight shifts in the timing of pri-let-7 expression between experiments indicate that expression of endogenous pri-let-7 transcripts is dynamic, and that even slight changes in culture conditions can affect the rate of development and thus pri-let-7 expression. GFP mRNA levels of the let-7 promoter reporter oscillated with a frequency identical to that of endogenous pri-let-7 expression, suggesting that transcriptional mechanisms largely control the cycling pattern of pri-let-7 expression. Consistent with previous reports, pre- and mature let-7 RNAs were undetectable until the L3 stage. In the L3 and L4 stages, levels of pre-let-7 oscillated in parallel to those of pri-let-7, while mature let-7 accumulated to a relatively constant level. | Primary Identifier | Expr9613 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00002285 | let-7 | C05G5.6 | Caenorhabditis elegans |
